Anti-BAF53 (W237) ACTL6A Antibody

Product Name

Anti-BAF53 (W237) ACTL6A Antibody

View all BAF53A Antibodies

SKU/Catalog Number

A05553

Size

100ul

Form

Liquid

Description

Boster Bio Anti-BAF53 (W237) ACTL6A Antibody catalog # A05553. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BAF53 (W237) ACTL6A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05553)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MSK2 around the phosphorylation site of T568.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05553 is reactive to ACTL6A in Human, Mouse, Rat

Applications

A05553 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

47.461kDa

Background of BAF53A

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.

Pierrat B.,J. Biol. Chem. 273:29661-29671(1998).
Deak M.,EMBO J. 17:4426-4441(1998).
Soloaga A.,EMBO J. 22:2788-2797(2003).<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of ACTL6A using Anti-BAF53 (W237) ACTL6A Antibody (A05553).

Immunohistochemistry (IHC) analyzes of BAF53 (W237) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-BAF53 (W237) ACTL6A Antibody A05553

Western blot (WB) analysis of BAF53 (W237) pAb at 1:500 dilution
Lane1:PC3 whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:The Uterus tissue lysate of Rat(40ug)
Lane4:The Uterus tissue lysate of Mouse(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For ACTL6A (Source: Uniprot.org, NCBI)

Alternative Names

actin-like 6A; actin-like protein 6A; actin-related protein 4,53 kDa BRG1-associated factor A; Actin-related protein Baf53a; Actl6; Arp4; arpNbeta; BAF complex 53 kDa subunit; Baf53a; BAF53AACTL6; BAF53MGC5382; BRG1-associated factor 53A; hArpN beta; INO80 complex subunit K; INO80KARPN-BETA ACTL6A ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K actin like 6A actin-like protein 6A|53 kDa BRG1-associated factor A|BAF complex 53 kDa subunit|BAF53|BRG1-associated factor 53A|INO80 complex subunit K|actin-related protein 4|actin-related protein Baf53a|arpNbeta|hArpN beta

For more info on ACTL6A, check out the ACTL6A Infographic

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In this infographic, you will see the following information for ACTL6A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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