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Product Info Summary
| SKU: | A02734-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-BANF1 Antibody
SKU/Catalog Number
A02734-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BANF1 Antibody (Catalog # A02734-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BANF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BANF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02734-1)
Host
Rabbit
Contents
BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-BANF1 antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human BANF1. The immunogen is located within the first 50 amino acids of BANF1.
Reactive Species
A02734-1 is reactive to BANF1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
10.1 kDa
Background of BANF1
Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02734-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Lines
Loading: 15 μg of lysates per lane.
Antibodies: BANF1, 4017 (2 μg/mL), BANF1, A02734-1 (2 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in HeLa Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: BANF1 A02734-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: BANF1, A02734-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: BANF1, A02734-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of BANF1 in Human Kideny Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with A02734-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-BANF1 Antibody (A02734-1)
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