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Product Info Summary
| SKU: | A03767 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-BAP31 BCAP31 Antibody
SKU/Catalog Number
A03767
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BAP31 BCAP31 Antibody (Catalog # A03767). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BAP31 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BAP31 BCAP31 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03767)
Host
Rabbit
Contents
BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-BAP31 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BAP31. The immunogen is located within the last 50 amino acids of BAP31.
Reactive Species
A03767 is reactive to BCAP31 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.0 kDa
Background of BCAP31
Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. It may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopicially, is also a potent inducer cell death.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03767 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC: 2 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Ramos Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: BAP31, A03767 (A: 0.5 μg/mL, B: 1 μg/mL and C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: BAP31, A03767 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Rat Lung Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: BAP31, A03767 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of BAP31 in Rat Lung Tissue
Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-BAP31 antibody (A03767) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-BAP31 BCAP31 Antibody (A03767)
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