Anti-BAT1 Antibody (C-term)

Product Name

Anti-BAT1 Antibody (C-term)

View all UAP56 Antibodies

SKU/Catalog Number

A03490-1

Size

80 µl

Description

Boster Bio Anti-BAT1 Antibody (C-term) (Catalog # A03490-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-BAT1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03490-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This BAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the C-terminal region of human BAT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03490-1 is reactive to DDX39B in Human

Applications

A03490-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

48.991kDa

Background of UAP56

Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. It has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. It can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. It can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50

Validation Images & Assay Conditions

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BAT1 Antibody (C-term) western blot analysis in A431,Hela,Jurkat cell line lysates (35ug/lane). This demonstrates the BAT1 antibody detected the BAT1 protein (arrow).

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All lanes : Anti-BAT1 Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 49 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Formalin-fixed and paraffin-embedded human kidney reacted with BAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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BAT1 Antibody (C-term) (Cat. #A03490-1) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Gene/Protein Information For DDX39B (Source: Uniprot.org, NCBI)

Alternative Names

56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B; DEAD box protein UAP56; EC 3.6.4.13; HLA-B associated transcript 1; HLA-B-associated transcript 1 protein; spliceosome RNA helicase BAT1; UAP56D6S81EBAT1nuclear RNA helicase (DEAD family) DDX39B BAT1, D6S81E, UAP56 DExD-box helicase 39B spliceosome RNA helicase DDX39B|56 kDa U2AF65-associated protein|ATP-dependent RNA helicase p47|DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B|DEAD-box helicase 39B|HLA-B-associated transcript 1 protein|nuclear RNA helicase (DEAD family)|spliceosome RNA helicase BAT1

For more info on DDX39B, check out the DDX39B Infographic

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In this infographic, you will see the following information for DDX39B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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