Product Info Summary
| SKU: | A04089 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-BATF Antibody
SKU/Catalog Number
A04089
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BATF Antibody (Catalog # A04089). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BATF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BATF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04089)
Host
Rabbit
Contents
BATF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BATF antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BATF. The immunogen is located within the first 50 amino acids of BATF.
Cross-reactivity
Multiple isoforms of BATF are known to exist. Despite its predicted molecular weight, BATF often migrates at a higher than expected molecular weight.
Reactive Species
A04089 is reactive to BATF in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
14.1 kDa
Background of BATF
BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04089 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BATF antibody can be used for detection of BATF by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.
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Immunofluorescence of BATF in human spleen tissue with BATF antibody at 20 μg/mL.
Specific Publications For Anti-BATF Antibody (A04089)
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