Click image to see more details
Product Info Summary
| SKU: | M03640 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-BBS4 Antibody (Center)
SKU/Catalog Number
M03640
Size
80 µl
Description
Boster Bio Anti-BBS4 Antibody (Center) (Catalog # M03640). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BBS4 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03640)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1292CT845.130.218
Isotype
IgG1
Immunogen
This BBS4 antibody is generated from a mice immunized with a recombinant protein between 1-240 amino acids from human BBS4.
Reactive Species
M03640 is reactive to BBS4 in Human, Mouse, Rat
Calculated molecular weight
58.3 kDa
Background of BBS4
The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03640 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 1:250
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1. M03640 was diluted at 1:250 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1. M03640 was diluted at 1:250 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary
Click image to see more details
Western blot analysis of lysates from Hela, U251 cell line, mouse heart and kidney, rat heart and kidney tissue (from left to right), using PHB Antibody . M03640 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Specific Publications For Anti-BBS4 Antibody (Center) (M03640)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-BBS4 Antibody (Center)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-BBS4 Antibody (Center)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
