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Product Info Summary
| SKU: | A13963-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BCAS4 Antibody
SKU/Catalog Number
A13963-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BCAS4 Antibody (Catalog # A13963-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
BCAS4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BCAS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13963-1)
Host
Rabbit
Contents
BCAS4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BCAS4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human BCAS4. The immunogen is located within the first 50 amino acids of BCAS4.
Reactive Species
A13963-1 is reactive to BCAS4 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
22.8 kDa
Background of BCAS4
The Breast carcinoma amplified sequence (BCAS4) gene is localized to 20q13, a region that is often amplified in breast cancers. It was isolated by the cloning of a highly overexpressed 1.3 kb transcript from the fusion of the last two exons of the BCAS3 and BCAS4 genes. This BCAS3-BCAS4 fusion transcript is only found in the MCF7 cell line, but the BCAS4 gene is also overexpressed in nine of thirteen breast cancer cell lines. The role of BCAS4 is unknown but its mRNA is highly expressed in thymus, spleen, kidney, brain, and placenta, suggesting BCAS4 plays other roles in multiple tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13963-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BCAS4 antibody can be used for detection of BCAS4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BCAS4 in 3T3 cell lysate with BCAS4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of BCAS4 in human breast carcinoma with BCAS4 antibody at 5 μg/mL.
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Immunofluorescence of BCAS4 in human breast carcinoma tissue with BCAS4 antibody at 20 μg/mL.
Specific Publications For Anti-BCAS4 Antibody (A13963-1)
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