Anti-BCKD BCKDK Antibody

Product Name

Anti-BCKD BCKDK Antibody

View all BCKDK Antibodies

SKU/Catalog Number

A07798

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-BCKD BCKDK Antibody (Catalog # A07798). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BCKD BCKDK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07798)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from Internal of human BCKD.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07798 is reactive to BCKDK in Human, Mouse, Rat

Applications

A07798 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46.36kDa

Background of BCKDK

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex By similarity.

Chuang J.C., Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Western blot analysis of extracts from 293 cells and Jurkat cells, using BCKD antibody A07798.

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Immunofluorescence analysis of HeLa cells, using BCKD antibody A07798.

Gene/Protein Information For BCKDK (Source: Uniprot.org, NCBI)

Alternative Names

BCKDHKIN; branched chain alpha-ketoacid dehydrogenase kinase; branched chain ketoacid dehydrogenase kinase; EC 2.7.11; EC 2.7.11.4; mitochondrial BCKDK BCKDKD, BDK branched chain keto acid dehydrogenase kinase [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial|3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial|BCKD-kinase|BCKDHKIN|branched chain alpha-ketoacid dehydrogenase kinase|branched chain ketoacid dehydrogenase kinase

For more info on BCKDK, check out the BCKDK Infographic

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In this infographic, you will see the following information for BCKDK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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