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Product Info Summary
| SKU: | M00047-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-MECP2 Antibody Picoband® (monoclonal, 2G3)
SKU/Catalog Number
M00047-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MECP2 Antibody Picoband® (monoclonal, 2G3) catalog # M00047-3. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MECP2 Antibody Picoband® (monoclonal, 2G3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00047-3)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
2G3
Isotype
Mouse IgG1
Immunogen
E.coli-derived human MECP2 recombinant protein (Position: K119-R453).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00047-3 is reactive to MECP2 in Human
Observed Molecular Weight
75 kDa
Calculated molecular weight
52.4 kDa
Background of MECP2
MECP2 (methyl CpG binding protein 2) is agenethat encodes theproteinMECP2. It is mapped to Xq28. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00047-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U87 whole cell, human Hela whole cell, human K562 whole cell, human HEK293 whole cell, human PC-3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MECP2 using anti-MECP2 antibody (M00047-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MECP2 antigen affinity purified monoclonal antibody (Catalog # M00047-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MECP2 at approximately 75KD. The expected band size for MECP2 is at 75KD.
Specific Publications For Anti-MECP2 Antibody Picoband® (monoclonal, 2G3) (M00047-3)
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