BCKDK Antibodies

AND BCKDK ELISA kits, BCKDK Proteins

Many biological assays use antibodies to detect BCKDK, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with BCKDK in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop BCKDK antibodies...

We validate the specificity of these antibodies to BCKDK by testing them on tissues known to express BCKDK positively and negatively. Browse below to find the BCKDK antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Previous: CCN4 Next: MAEA

BCKDK Infographic by Boster Bio

All BCKDK Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-BCKDK (E41) Antibody

Western blot (WB) analysis of BCKDK (E41) polyclonal antibody at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:AML-12 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:L02 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of BCKDK (E41) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50

Immunoprecipitation of HEK293T cell lysate using BCKDK (E41) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 and lane 5)

Human, Mouse, Rat

IP, IHC, WB

$405
Cat # A07798-1
100ul

Anti-BCKDH kinase (BCKDK) Mouse Monoclonal Antibody [Clone ID: OTI11C9]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-BCKDK mouse monoclonal antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BCKDK mouse monoclonal antibody

Human, Mouse, Rat

IF, IHC, WB

$523
Cat # M07798
100 µl

Anti-BCKDK Antibody Picoband®

Western blot analysis of BCKDK using anti-BCKDK antibody (A07798-2)

IHC analysis of BCKDK using anti-BCKDK antibody (A07798-2)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A07798-2
100 μg/vial

Anti-BCKDK Rabbit Monoclonal Antibody

Western blot analysis of BCKDK expression in HeLa cell lysate

Human, Mouse, Rat

$370
Cat # M07798-1
100 μl/vial

BCKDH kinase (BCKDK) (NM_005881) Human Over-expression Lysate

Human

$628
Cat # LS010295
20 µg, 100 µg

BCKDK Overview

Facts about [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial.

BCKDK 3D structure. Source: alphafold

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial (BCKDK)

Catalyzes the phosphorylation and inactivation of this branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that govern the activity state of the BCKD complex.

Gene Information

Human
Gene Name:	BCKDK
Uniprot:	O14874
Entrez:	10295

Family

Belongs to:

PDK/BCKDK protein kinase family

Alternative Names

BCKDHKIN; branched chain alpha-ketoacid dehydrogenase kinase; branched chain ketoacid dehydrogenase kinase; EC 2.7.11; EC 2.7.11.4; mitochondrial

Molecular Weight

Mass (kDA):
46.36 kDA

Genomic Context


Human

Location:	16p11.2
Sequence:	16; NC_000016.10 (31108381..31117651)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Mitochondrion matrix. Mitochondrion.

Diseases

Epilepsy
Autistic Disorder
Impairment (finding)
Autism Spectrum Disorders
Diabetes Mellitus
Dysequilibrium Syndrome

Interacting Proteins

STAT3

PTMs

Phosphorylation

Research Areas

Protein Kinase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BCKDK

References

PMID: 22956686 by Novarino G., et al. Mutations in BCKD-kinase lead to a potentially treatable form of autism with epilepsy.

PMID: 24449431 by Garcia-Cazorla A., et al. Two novel mutations in the BCKDK (branched-chain keto-acid dehydrogenase kinase) gene are responsible for a neurobehavioral deficit in two pediatric unrelated patients.