BCKDK Antibodies

AND BCKDK ELISA kits, BCKDK Proteins

Many biological assays use antibodies to detect BCKDK, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with BCKDK in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop BCKDK antibodies...

We validate the specificity of these antibodies to BCKDK by testing them on tissues known to express BCKDK positively and negatively. Browse below to find the BCKDK antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for BCKDK? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the BCKDK IHC Guide

BCKDK Infographic by Boster Bio

All BCKDK Products

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Product

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Specificity

Applications

Price

Anti-BCKDK Antibody Picoband®

Western blot analysis of BCKDK using anti-BCKDK antibody (A07798-2)

IHC analysis of BCKDK using anti-BCKDK antibody (A07798-2)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A07798-2
100 μg/vial

Anti-BCKDK Rabbit Monoclonal Antibody

Western blot analysis of BCKDK expression in HeLa cell lysate

Human, Mouse, Rat

$370
Cat # M07798-1
100 μl/vial

BCKDH kinase (BCKDK) (NM_005881) Human Over-expression Lysate

Human

$628
Cat # LS010295
20 µg, 100 µg

BCKDK Overview

Facts about [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial.

BCKDK 3D structure. Source: alphafold

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial (BCKDK)

Catalyzes the phosphorylation and inactivation of this branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that govern the activity state of the BCKD complex.

Gene Information

Human
Gene Name:	BCKDK
Uniprot:	O14874
Entrez:	10295

Family

Belongs to:

PDK/BCKDK protein kinase family

Alternative Names

BCKDHKIN; branched chain alpha-ketoacid dehydrogenase kinase; branched chain ketoacid dehydrogenase kinase; EC 2.7.11; EC 2.7.11.4; mitochondrial

Molecular Weight

Mass (kDA):
46.36 kDA

Genomic Context


Human

Location:	16p11.2
Sequence:	16; NC_000016.10 (31108381..31117651)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Mitochondrion matrix. Mitochondrion.

Diseases

Epilepsy
Autistic Disorder
Impairment (finding)
Autism Spectrum Disorders
Diabetes Mellitus
Dysequilibrium Syndrome

Interacting Proteins

STAT3

PTMs

Phosphorylation

Research Areas

Protein Kinase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BCKDK

References

PMID: 22956686 by Novarino G., et al. Mutations in BCKD-kinase lead to a potentially treatable form of autism with epilepsy.

PMID: 24449431 by Garcia-Cazorla A., et al. Two novel mutations in the BCKDK (branched-chain keto-acid dehydrogenase kinase) gene are responsible for a neurobehavioral deficit in two pediatric unrelated patients.