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BCL2 antibody (A00040-2) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 161 Publications

a00040 2 bcl2 primary antibodies fcm testing 3

Flow Cytometry analysis of U20S cells using anti-BCL2 antibody (A00040-2).
Overlay histogram showing U20S cells stained with A00040-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL2 Antibody (A00040-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Bcl-2/BCL2 Antibody Picoband®

BCL2 antibody

161 Publications

Reactive Species	Host	Application
Human	Rabbit	ELISA, Flow Cytometry, IF, ICC, WB

Tested Applications

BCL2 antibody was positively detected by WB in:

human Jurkat whole cell, human HL-60 whole cell, human THP-1 whole cell, huamn OE19 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

BCL2 antibody was positively detected by ICC/IF in:

U20S cell

BCL2 antibody was positively detected by FC in:

U20S cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ELISA

Human

Tested

Recommend

Mouse

Predicted

Rat

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-Bcl-2/BCL2 Antibody Picoband®
View all BCL2 Antibodies

Form

Lyophilized

Uniprot

P10415

Gene ID

596

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Bcl-2 recombinant protein (Position: Q118-E165).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

26 kDa

Calculated Molecular Weight

26.3 kDa

Background of BCL2

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Cite This Product

Anti-Bcl-2/BCL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00040-2)

Protocols

Western Blot protocol for BCL2 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for BCL2 antibody

Review Protocol

Flow Cytometry (FC) protocol for BCL2 antibody

Review Protocol

Publications

Prolonged sevoflurane exposure induces exaggerated and persistent cognitive decline in old rats with pre-existing impairment by increasing hippocampal mitochondrial dysfunction and apoptosis

Published: 2026-07-31

Journal: NEUROTOXICOLOGY

Application: IHC

Impact Factor: 3.80

Species: Rat

PMID: 42537810

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Free Secondary Antibody

Anti-Bcl-2/BCL2 Antibody Picoband®

BCL2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Prolonged sevoflurane exposure induces exaggerated and persistent cognitive decline in old rats with pre-existing impairment by increasing hippocampal mitochondrial dysfunction and apoptosis

Sodium butyrate induces mitochondrial pathway apoptosis in liver cancer via ATF4/SLC7A11-mediated ferroptosis

CHMP2B exacerbates renal tubular injury in sepsis-induced acute kidney injury by enhancing autophagy blockade

Repurposing Lamivudine: A novel therapeutic strategy for ischemic stroke targeting the glycolytic enzyme Pgk1

Screening potential therapeutic drugs for lung adenocarcinoma based on hypoxia and pyrimidine metabolism models

Inhibition of PGK1 ameliorates acute kidney injury through inactivating the PKM2/ALOX12/ferroptosis pathway in a study with male mice

miR-107 suppresses porcine granulosa cell proliferation and estradiol synthesis while promoting apoptosis via targeting PTGS2

High expression of ARPC1B promotes the proliferation and apoptosis of clear cell renal cell carcinoma cells, leading to a poor prognosis

Bufadienolides from Chansu Injection Synergistically Enhances the Antitumor Effect of Erlotinib by Inhibiting the KRAS Pathway in Pancreatic Cancer

Astragaloside IV plays a neuroprotective role by promoting PPARγ in cerebral ischemia-reperfusion rats

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability

Engineering of phytic acid-coated Prussian nanoparticles for combined Nitric oxide and Low-Temperature photothermal therapy of osteosarcoma

Protopanaxadiol Targeting Membrane Induces HepG2 Cell Apoptosis Via Raft-like Formation and Tubulation Disruption

Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity

Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis

Rutaecarpine Ameliorates Murine N-Methyl-N’-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis by Sonic Hedgehog Pathway

Modified CFBP-bFGF targeting to ischemic brain promoted the functional recovery of cerebral ischemia

Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells

Metformin inhibits the tumor-promoting effect of low-dose resveratrol, and enhances the anti-tumor activity of high-dose resveratrol by increasing its reducibility in triple negative breast cancer

Using PCG-Arginine nanoparticle mediated intranasal delivery of dynorphin A (1–8) to improve neuroprotection in MCAO rats

TRB3 mediates vascular remodeling by activating the MAPK signaling pathway in hypoxic pulmonary hypertension

Tunicamycin Induces Hepatic Stellate Cell Apoptosis Through Calpain-2/Ca2 +-Dependent Endoplasmic Reticulum Stress Pathway

SIRT1/PGC-1α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells

CircHIPK3 regulates the autophagy and apoptosis of hypoxia/reoxygenation-stimulated cardiomyocytes via the miR-20b-5p/ATG7 axis

Protective Effect of GM1 Attenuates Hippocampus and Cortex Apoptosis After Ketamine Exposure in Neonatal Rat via PI3K/AKT/GSK3β Pathway

Insulin injections inhibits PTZ-induced mitochondrial dysfunction, oxidative stress and neurological deficits via the SIRT1/PGC-1α/SIRT3 pathway

Continuous artificial light at night exacerbates diisononyl phthalate-induced learning and memory impairment in mice: Toxicological evidence

Dietary Calcium Alleviates Fluorine-Induced Liver Injury in Rats by Mitochondrial Apoptosis Pathway

Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway

Aptamer-protamine-siRNA nanoparticles in targeted therapy of ErbB3 positive breast cancer cells

CircPOSTN/miR-361-5p/TPX2 axis regulates cell growth, apoptosis and aerobic glycolysis in glioma cells

COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice

Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway

Antioxidant preconditioning improves therapeutic outcomes of adipose tissue-derived mesenchymal stem cells through enhancing intrahepatic engraftment efficiency in a mouse liver fibrosis model

Icariin improves cognitive deficits by reducing the deposition of β-amyloid peptide and inhibition of neurons apoptosis in SAMP8 mice

[6]-Gingerol Ameliorates ISO-Induced Myocardial Fibrosis by Reducing Oxidative Stress, Inflammation, and Apoptosis through Inhibition of TLR4/MAPKs/NF-κB Pathway

Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro

Lactobacillus rhamnosus FLRH93 protects against intestinal damage in mice induced by 5-fluorouracil

Neuroprotective effects of miR-532-5p against ischemic stroke

Polysaccharide from the seeds of Plantago asiatica L. alleviates nonylphenol induced reproductive system injury of male rats via PI3K/Akt/mTOR pathway

Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats

Schizandrol A protects against Aβ1–42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons

MiR-199a-5p regulates rat liver regeneration and hepatocyte proliferation by targeting TNF-α TNFR1/TRADD/CASPASE8/CASPASE3 signalling pathway

Effects of rosmarinic acid on immunoregulatory activity and hepatocellular carcinoma cell apoptosis in H22 tumor-bearing mice

The study of effect and mechanism of 630-nm laser on human lung adenocarcinoma cell xenograft model in nude mice mediated by hematoporphyrin derivatives

MiR-183-5p protects rat hearts against myocardial ischemia/reperfusion injury through targeting VDAC1

Protective effects of pentadecapeptide derived from Cyclaina sinensis against cyclophosphamide-induced hepatotoxicity

Elimination of Intracellular Calcium Overload by BAPTA-AM-Loaded Liposomes: A Promising Therapeutic Agent for Acute Liver Failure

LaSota Strain Expressing The Rabies Virus Glycoprotein (rL-RVG) Suppresses Gastric Cancer by Inhibiting the Alpha 7 Nicotinic Acetylcholine Receptor (α7 nAChR)/Phosphoinositide 3-Kinase (PI3K)/AKT Pathway

Preoperative vitamin-rich carbohydrate loading alleviates postoperative cognitive dysfunction in aged rats

Inotodiol inhibits cells migration and invasion and induces apoptosis via p53-dependent pathway in HeLa cells

Alteronol Enhances the Anti-tumor Activity and Reduces the Toxicity of High-Dose Adriamycin in Breast Cancer

Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway

Treatment with MQA, a Derivative of Caffeoylquinic Acid, Provides Neuroprotective Effects against Cerebral Ischemia Through Suppression of the p38 Pathway and Oxidative Stress in Rats

Perfluorooctanoic acid exposure during pregnancy alters the apoptosis of uterine cells in pregnant mice

Noncoding transcribed ultraconserved region (T-UCR) UC.48+ is a novel regulator of high-fat diet induced myocardial ischemia/reperfusion injury

The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways

Role of capsaicin sensitive sensory nerves in ischemia reperfusion-induced acute kidney injury in rats

Platycodin D protects acetaminophen-induced hepatotoxicity by inhibiting hepatocyte MAPK pathway and apoptosis in C57BL/6J mice

Insight into the negative impact of ionic liquid: A cytotoxicity mechanism of 1-methyl-3-octylimidazolium bromide

Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII

Canonical Transient Receptor Potential Channel 3 Contributes to Febrile Seizure Inducing Neuronal Cell Death and Neuroinflammation

Lithium chloride (LiCl) induced autophagy and downregulated expression of transforming growth factor β-induced protein (TGFBI) in granular corneal dystrophy

The impact of acute thermal stress on green mussel Perna viridis: Oxidative damage and responses

Acute downregulation of miR-199a attenuates sepsis-induced acute lung injury by targeting SIRT1

Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats

PSMB8 regulates glioma cell migration, proliferation, and apoptosis through modulating ERK1/2 and PI3K/AKT signaling pathways