Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)

Boster Bio Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) catalog # MA1004. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Cited in 163 publication(s).

Product Info Summary

SKU: MA1004
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, ICC, WB

Product Name

Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)

See all Bcl-2 products

SKU/Catalog Number

MA1004

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) catalog # MA1004. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1004)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

Bcl-2-100

Isotype

Mouse IgG1

Immunogen

Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1004 is reactive to Bcl2 in Human

Applications

MA1004 is guaranteed for IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, -
Western blot, 1-2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Bcl2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P49950

Gene ID

24224

Gene Name

Bcl2

Full Name

Apoptosis regulator Bcl-2

Weight

26.622kDa

Superfamily

Bcl-2 family

Alternative Names

apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2 Bcl2|Bcl-2|BCL2, apoptosis regulator|apoptosis regulator Bcl-2|B cell lymphoma 2 associated oncogene|B-cell CLL/lymphoma 2|B-cell leukemia/lymphoma 2|Bcl-2alpha|Bcl2-like protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bcl2, check out the Bcl2 Infographic

Bcl2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bcl2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1004 has been cited in 163 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Oleanolic acid from Prunella Vulgaris L. Induces SPC-A-1 cell line apoptosis via regulation of Bax, Bad and Bcl-2 Expression

Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress, inflammation and sonic hedgehog pathway in rats

Antitumor effects of Laminaria extract fucoxanthin on lung cancer

Recombinant Newcastle disease virus expressing human IFN%u2010%u03BB1 (rL%u2010hIFN%u2010%u03BB1)%u2010induced apoptosis of A549 cells is connected to endoplasmic reticulum stress %u2026

The inhibition effect of triptolide on human endometrial carcinoma cell line HEC-1B: A in vitro and in vivo studies

Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes

NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway

Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling

Reactive oxygen species-induced apoptosis in PC12 cells and protective effect of bilobalide

Myricetin exhibits anti-glioma potential by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and ROS generation

Copper ion attenuated the antiproliferative activity of di-2-pyridylhydrazone dithiocarbamate derivative; however, there was a lack of correlation between ROS %u2026

Laminarin%u2011induced apoptosis in human colon cancer LoVo cells

Reactive oxygen species mediate isoalantolactone-induced apoptosis in human prostate cancer cells

Ginsenoside-Rh2 inhibits proliferation and induces apoptosis of human gastric cancer SGC-7901 side population cells

Inhibition of cpu0213, a dual endothelin receptor antagonist, on apoptosis via nox4-dependent ros in hk-2 cells

Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by %u2026

Correlation of acetabular chondrocyte apoptosis with caspase-3 and BCL-2 expression in developmental dislocations of the hip

Inhibitory effects of vitamin E on osteocyte apoptosis and DNA oxidative damage in bone marrow hemopoietic cells at early stage of steroid-induced femoral %u2026

Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells

N-acetylcysteine reduces oxidative stress, nuclear factor-?B activity and cardiomyocyte apoptosis in heart failure

Fibroblast growth factor 21 protects rat cardiomyocytes from endoplasmic reticulum stress by promoting the fibroblast growth factor receptor 1-extracellular signal-regulated kinase 1/2 signaling pathway

Ailanthone induces G2/M cell cycle arrest and apoptosis of SGC-7901 human gastric cancer cells

1-calcium phosphate-uracil, a synthesized pyrimidine derivative agent, has anti-proliferative, pro-apoptotic and anti-invasion effects on multiple tumor cell lines

CCAAT/enhancer binding protein homologous protein knockdown alleviates hypoxia-induced myocardial injury in rat cardiomyocytes exposed to high glucose

Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice

Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils

Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells

Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway

Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

RKTG overexpression inhibits proliferation and induces apoptosis of human leukemia cells via suppression of the ERK and PI3K/AKT signaling pathways

Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure

Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction

Vanadium toxicity in the thymic development

Comparative Study on the Protective Effects of Salidroside and Hypoxic Preconditioning for Attenuating Anoxia-Induced Apoptosis in Pheochromocytoma (PC12) Cells

Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis

The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats

Effects of As2O3nanoparticles on cell growth and apoptosis of NB4 cells

Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats

Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer.

Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy

A preliminary study of the effect of ECRG4 overexpression on the proliferation and apoptosis of human laryngeal cancer cells and the underlying mechanisms

Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuatedSalmonellaserovartyphimuriumfor prostate cancer treatment

Expression of Survivin in pancreatic cancer and its correlation to expression of Bcl-2

In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Effects of garlicin on apoptosis in rat model of colitis

Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells

Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine

A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway

Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury

Astaxanthin Inhibits Acetaldehyde-Induced Cytotoxicity in SH-SY5Y Cells by Modulating Akt/CREB and p38MAPK/ERK Signaling Pathways

Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo

Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway

Mesenchymal stem cells promote colorectal cancer progression through AMPK/mTOR-mediated NF-?B activation

Yangjing Capsule Ameliorates Spermatogenesis in Male Mice Exposed to Cyclophosphamide

Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB

Hippocampal mitogen-activated protein kinase activation is associated with intermittent hypoxia in a rat model of obstructive sleep apnea syndrome

Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance

Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats

Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats.

Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms

Wang Y, Li Y, Yang W, Gao S, Lin J, Wang T, Zhou K, Hu H. Am J Transl Res. 2018 Mar 15;10(3):796-805. eCollection 2018. Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer’s disease induced by Aβ1-40

Qi QM, Xue YC, Lv J, Sun D, Du JX, Cai SQ, Li YH, Gu TC, Wang MB. Oncol Lett. 2018 May;15(5):6400-6408. doi: 10.3892/ol.2018.8177. Epub 2018 Mar 5. Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochond...

Yang H, Wang C, Chen H, Li L, Ma S, Wang H, Fu Y, Qu T. Stem Cells Int. 2018 Apr 3;2018:4659159. doi: 10.1155/2018/4659159. eCollection 2018. Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats

Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...

Du F, Ding Y, Zou J, Li Z, Tian J, She R, Wang D, Wang H, Lv D, Chang L. Plos One. 2015 May 22;10(5):E0127047. Doi: 10.1371/Journal.Pone.0127047. Ecollection 2015. Morphology And Molecular Mechanisms Of Hepatic Injury In Rats Under Simulated Weigh...

Li Tt, Zhang Jf, Fei Sj, Zhu Sp, Zhu Jz, Qiao X, Liu Zb. Acta Pharmacol Sin. 2014 Feb;35(2):185-94. Doi: 10.1038/Aps.2013.140. Epub 2013 Dec 23. Glutamate Microinjection Into The Hypothalamic Paraventricular Nucleus Attenuates Ulcerative Colitis I...

Chen Xl, Cao Lq, She Mr, Wang Q, Huang Xh, Fu Xh. World J Gastroenterol. 2008 Jan 28;14(4):582-9. Gli-1 Sirna Induced Apoptosis In Huh7 Cells.

Qin J, Liu M, Ding Q, Ji X, Hao Y, Wu X, Xiong J. Mol Cell Biochem. 2014 Oct;395(1-2):99-107. Doi: 10.1007/S11010-014-2115-2. Epub 2014 Jun 17. The Direct Effect Of Estrogen On Cell Viability And Apoptosis In Human Gastric Cancer Cells.

Lan Z, Liu J, Chen L, Fu Q, Luo J, Qu R, Kong L, Ma S. J Ethnopharmacol. 2012 May 7;141(1):386-95. Doi: 10.1016/J.Jep.2012.02.050. Epub 2012 Mar 5. Danggui-Shaoyao-San Ameliorates Cognition Deficits And Attenuates Oxidative Stress-Related Neuronal...

Sun W, Liu J, Huan Y, Zhang C. Inflamm Res. 2014 Apr;63(4):287-97. Doi: 10.1007/S00011-013-0699-8. Epub 2013 Dec 19. Intracranial Injection Of Recombinant Stromal-Derived Factor-1 Alpha (Sdf-1??) Attenuates Traumatic Brain Injury In Rats.

Yao K, Xing H, Yang W, Liao A, Wu B, Li Y, Zhang R, Liu Z. Tumour Biol. 2014 Aug;35(8):8023-31. Doi: 10.1007/S13277-014-2073-Z. Epub 2014 May 18. Knockdown Of Rlip76 Expression By Rna Interference Inhibits Proliferation, Enhances Apoptosis, And In...

Li X, Huo X, Zhang C, Ma X, Han F, Wang G. Cell Physiol Biochem. 2014;34(4):1227-40. Doi: 10.1159/000366334. Epub 2014 Sep 25. Role Of Continuous High Thoracic Epidural Anesthesia In Hippocampal Apoptosis After Global Cerebral Ischemia In Rats.

Gao H, Jiang Q, Han Y, Peng J, Wang C. Cell Biochem Biophys. 2015 Mar;71(2):827-35. Doi: 10.1007/S12013-014-0270-4. Shrna-Mediated Emmprin Silencing Inhibits Human Leukemic Monocyte Lymphoma U937 Cell Proliferation And Increases Chemosensitivity T...

Gao H, Jiang Q, Han Y, Peng J, Wang C. Cell Biochem Biophys. 2015 Mar;71(2):757-64. Doi: 10.1007/S12013-014-0260-6. Hispidulin Potentiates The Antitumor Effect Of Sunitinib Against Human Renal Cell Carcinoma In Laboratory Models.

Guan J, Li H, Lv T, Chen D, Yuan Y, Qu S. Biochem Biophys Res Commun. 2013 Nov 22;441(3):560-6. Doi: 10.1016/J.Bbrc.2013.10.121. Epub 2013 Oct 30. Bone Morphogenetic Protein-7 (Bmp-7) Mediates Ischemic Preconditioning-Induced Ischemic Tolerance Vi...

Gao H, Xie J, Peng J, Han Y, Jiang Q, Han M, Wang C. Exp Cell Res. 2015 Mar 15;332(2):236-46. Doi: 10.1016/J.Yexcr.2014.11.021. Epub 2014 Dec 8. Hispidulin Inhibits Proliferation And Enhances Chemosensitivity Of Gallbladder Cancer Cells By Targeti...

Zhou Pq, Fan H, Hu H, Tang Q, Liu Xx, Zhang Lj, Zhong M, Shou Zx. J Huazhong Univ Sci Technolog Med Sci. 2014 Dec;34(6):815-20. Doi: 10.1007/S11596-014-1358-1. Epub 2014 Dec 6. Role Of Dor-??-Arrestin1-Bcl2 Signal Transduction Pathway And Interven...

Tian L, Zhang J, Ge J, Xiao H, Lu J, Fu S, Liu M, Sun Y. Med Oncol. 2014 Jan;31(1):785. Doi: 10.1007/S12032-013-0785-3. Epub 2013 Dec 3. Microrna-205 Suppresses Proliferation And Promotes Apoptosis In Laryngeal Squamous Cell Carcinoma.

Rasul A, Khan M, Yu B, Ali M, Bo Yj, Yang H, Ma T. Arch Pharm Res. 2013 Oct;36(10):1262-9. Doi: 10.1007/S12272-013-0217-0. Epub 2013 Jul 24. Isoalantolactone, A Sesquiterpene Lactone, Induces Apoptosis In Sgc-7901 Cells Via Mitochondrial And Phosp...

Wang Py, Li Yj, Zhang S, Li Zl, Yue Z, Xie N, Xie Sy. Mol Cell Biochem. 2010 Jun;339(1-2):163-71. Doi: 10.1007/S11010-009-0380-2. Epub 2010 Jan 5. Regulating A549 Cells Growth By Aso Inhibiting Mirna Expression.

Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.

Chen J, Wei H, Xie B, Wang B, Cheng J, Cheng J. Leuk Res. 2012 Dec;36(12):1526-35. Doi: 10.1016/J.Leukres.2012.08.018. Epub 2012 Sep 7. Endoplasmic Reticulum Stress Contributes To Arsenic Trioxide-Induced Apoptosis In Drug-Sensitive And -Resistant...

Chu Sh, Ma Yb, Feng Df, Zhang H, Qiu Jh, Zhu Za. Oncol Lett. 2011 Nov;2(6):1139-1142. Epub 2011 Aug 4. Elevated Expression Of Solute Carrier Family 22 Member 18 Increases The Sensitivity Of U251 Glioma Cells To Bcnu.

Zhao B, Li X. Oncol Rep. 2014 Jun;31(6):2769-75. Doi: 10.3892/Or.2014.3126. Epub 2014 Apr 3. Altholactone Induces Reactive Oxygen Species-Mediated Apoptosis In Bladder Cancer T24 Cells Through Mitochondrial Dysfunction, Mapk-P38 Activation And Akt...

Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.

Huang C, Lin Y, Su H, Ye D. Neurochem Res. 2015 Jan;40(1):27-35. Doi: 10.1007/S11064-014-1461-5. Epub 2014 Oct 25. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress And Apoptosis In Pc12 Cell.

Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...

Chen Xm, Bai Y, Zhong Yj, Xie Xl, Long Hw, Yang Yy, Wu Sg, Jia Q, Wang Xh. Plos One. 2013 Nov 12;8(11):E79201. Doi: 10.1371/Journal.Pone.0079201. Ecollection 2013. Wogonin Has Multiple Anti-Cancer Effects By Regulating C-Myc/Skp2/Fbw7?? And Hdac1/...

Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Pro...

Chen P, Wang L, Li N, Liu Q, Ni J. Metallomics. 2013 May;5(5):541-50. Doi: 10.1039/C3Mt00002H. Epub 2013 Apr 16. Comparative Proteomics Analysis Of Sodium Selenite-Induced Apoptosis In Human Prostate Cancer Cells.

Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.

Sun Y, Xu Hj, Zhao Yx, Wang Lz, Sun Lr, Wang Z, Sun Xf. Evid Based Complement Alternat Med. 2013;2013:690164. Doi: 10.1155/2013/690164. Epub 2013 Mar 19. Crocin Exhibits Antitumor Effects On Human Leukemia Hl-60 Cells In Vitro And In Vivo.

Shuai H, Zhang J, Zhang J, Xie J, Zhang M, Yu Y, Zhang L. Endocr Res. 2011;36(1):25-34. Doi: 10.3109/07435800.2010.534753. Erythropoietin Protects Pancreatic ??-Cell Line Nit-1 Cells Against Cytokine-Induced Apoptosis Via Phosphatidylinositol 3-Ki...

Chen J, Huang Rb. Neurosciences (Riyadh). 2009 Oct;14(4):343-8. Protective Effect Of Yulangsan Polysaccharide On Focal Cerebral Ischemia/Reperfusion Injury In Rats And Its Underlying Mechanism.

Liu W, Zheng Q, Wang Y, Han X, Yuan L, Zhao M. Neurochem Res. 2015 Jan;40(1):70-80. Doi: 10.1007/S11064-014-1467-Z. Epub 2014 Nov 5. Transplantation Of Olfactory Ensheathing Cells Attenuates Acute Carbon Monoxide Poisoning-Induced Brain Damages In...

Yuan F, Dong P, Wang X, Fu X, Dai M, Zhang W. Exp Toxicol Pathol. 2013 Nov;65(7-8):1011-8. Doi: 10.1016/J.Etp.2013.02.004. Epub 2013 Mar 19. Toxicological Effects Of Cigarette Smoke On Ana-1 Macrophages In Vitro.

Luo Q, Li Z, Yan J, Zhu F, Xu Rj, Cai Yz. J Med Food. 2009 Aug;12(4):695-703. Doi: 10.1089/Jmf.2008.1232. Lycium Barbarum Polysaccharides Induce Apoptosis In Human Prostate Cancer Cells And Inhibits Prostate Cancer Growth In A Xenograft Mouse Mode...

Wu Cy, Tang Zh, Jiang L, Li Xf, Jiang Zs, Liu Ls. Mol Cell Biochem. 2012 Jan;359(1-2):347-58. Doi: 10.1007/S11010-011-1028-6. Epub 2011 Aug 17. Pcsk9 Sirna Inhibits Huvec Apoptosis Induced By Ox-Ldl Via Bcl/Bax-Caspase9-Caspase3 Pathway.

Rasul A, Yu B, Zhong L, Khan M, Yang H, Ma T. Oncol Rep. 2012 May;27(5):1481-7. Doi: 10.3892/Or.2012.1694. Epub 2012 Feb 21. Cytotoxic Effect Of Evodiamine In Sgc-7901 Human Gastric Adenocarcinoma Cells Via Simultaneous Induction Of Ap...

Li Y, Gu Wj, Liu Hl. Dig Dis Sci. 2012 Aug;57(8):2072-8. Doi: 10.1007/S10620-012-2132-5. Epub 2012 May 10. Induction Of Pancreatic Cancer Cell Apoptosis And Enhancement Of Gemcitabine Sensitivity By Rap80 Sirna.

Wang Y, Liu X, Zhang D, Chen J, Liu S, Berk M. Bmc Med. 2013 Feb 8;11:32. Doi: 10.1186/1741-7015-11-32. The Effects Of Apoptosis Vulnerability Markers On The Myocardium In Depression After Myocardial Infarction.

Gu Wj, Liu Hl. Anticancer Drugs. 2013 Jul;24(6):566-76. Doi: 10.1097/Cad.0B013E3283608Bc5. Induction Of Pancreatic Cancer Cell Apoptosis, Invasion, Migration, And Enhancement Of Chemotherapy Sensitivity Of Gemcitabine, 5-Fu, And Oxaliplatin By Hnr...

Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang Nc, Li L. Oncol Rep. 2013 Jun;29(6):2101-8. Doi: 10.3892/Or.2013.2375. Epub 2013 Apr 3. Ent-11??-Hydroxy-15-Oxo-Kaur-16-En-19-Oic-Acid Induces Apoptosis And Cell Cycle Arrest In Cne-2Z Nasopharyngeal C...

Qin R, Shen H, Cao Y, Fang Y, Li H, Chen Q, Xu W. Plos One. 2013 Oct 1;8(10):E76486. Doi: 10.1371/Journal.Pone.0076486. Ecollection 2013. Tetrandrine Induces Mitochondria-Mediated Apoptosis In Human Gastric Cancer Bgc-823 Cells.

Wei T, Tian W, Liu F, Xie G. Biochem Biophys Res Commun. 2014 May 16;447(4):666-71. Doi: 10.1016/J.Bbrc.2014.04.074. Epub 2014 Apr 19. Protective Effects Of Exogenous ??-Hydroxybutyrate On Paraquat Toxicity In Rat Kidney.

Wang Y, Zhang H, Chai F, Liu X, Berk M,,,,. Bmc Psychiatry. 2014 Dec 4;14:349. Doi: 10.1186/S12888-014-0349-X. The Effects Of Escitalopram On Myocardial Apoptosis And The Expression Of Bax And Bcl-2 During Myocardial Ischemia/Reperfusion In A Mode...

Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.

Han N, Yu L, Song Z, Luo L, Wu Y. Mol Med Rep. 2015 Jul;12(1):1098-106. Doi: 10.3892/Mmr.2015.3540. Epub 2015 Mar 24. Agmatine Protects M??ller Cells From High-Concentration Glucose-Induced Cell Damage Via N-Methyl-D-Aspartic Acid Receptor Inhibit...

Sun J, Zhang H, Guan L, Zhou H, Sun M. Int J Clin Exp Med. 2015 Jan 15;8(1):358-67. Ecollection 2015. Alpha-Lipoic Acid Attenuates Trinitrobenzene Sulfonic Acid-Induced Ulcerative Colitis In Mice.

Rasul A, Ding C, Li X, Khan M, Yi F, Ali M, Ma T. Apoptosis. 2012 Oct;17(10):1104-19. Doi: 10.1007/S10495-012-0742-1. Dracorhodin Perchlorate Inhibits Pi3K/Akt And Nf-??b Activation, Up-Regulates The Expression Of P53, And Enhances Apoptosis.

Yin W, Li B, Li X, Yu F, Cai Q, Zhang Z, Wang J, Zhang J, Zhou R, Cheng M, Gao H. J Cardiovasc Pharmacol. 2015 Jan;65(1):13-21. Doi: 10.1097/Fjc.0000000000000157. Critical Role Of Prohibitin In Endothelial Cell Apoptosis Caused By Glycated Low-Den...

Zhou Y, Tan X, Kuang W, Liu L, Wan L. Transl Res. 2012 Jun;159(6):464-72. Doi: 10.1016/J.Trsl.2011.09.007. Epub 2011 Oct 24. Erythromycin Ameliorates Cigarette-Smoke-Induced Emphysema And Inflammation In Rats.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.

Wang Jm, Xiao Bl, Zheng Jw, Chen Hb, Zou Sq. World J Gastroenterol. 2007 Jun 21;13(23):3171-5. Effect Of Targeted Magnetic Nanoparticles Containing 5-Fu On Expression Of Bcl-2, Bax And Caspase 3 In Nude Mice With Transplanted Human Liver Cancer.

Meng Qt, Xia Zy, Luo T, Cao C, Zhao B, Wu Y, Wu X, Chen X. Saudi Med J. 2012 Feb;33(2):139-45. Ligustrazine Attenuates Acute Lung Injury Induced By Blunt Chest Trauma.

Zhou Y, Li W, Xu Q, Huang Y. J Exp Clin Cancer Res. 2010 Oct 4;29:131. Doi: 10.1186/1756-9966-29-131. Elevated Expression Of Dickkopf-1 Increases The Sensitivity Of Human Glioma Cell Line Shg44 To Bcnu.

Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...

Peng X, Chen K, Chen J, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W. Environ Toxicol. 2015 Mar 4. Doi: 10.1002/Tox.22120. [Epub Ahead Of Print] Aflatoxin B1 Affects Apoptosis And Expression Of Bax, Bcl-2, And Caspase-3 In Thymus And Bursa ...

Chen J, Li X, Qiu J, You H, Zhang Q, Dong G, Zuo Y. Cell Biochem Biophys. 2013;67(3):1015-9. Doi: 10.1007/S12013-013-9597-5. Kinetics Of Apoptosis And Expression Of Apoptosis-Related Proteins In Rat Ca3 Hippocampus Cells After Experimental Diffuse...

Liu Y, Zhou Y, Wang Y, Geng D, Liu J. Obes Surg. 2011 Sep;21(9):1424-31. Doi: 10.1007/S11695-011-0388-Z. Roux-En-Y Gastric Bypass-Induced Improvement Of Glucose Tolerance And Insulin Resistance In Type 2 Diabetic Rats Are Mediated By Glucagon-Like...

Yang Y, Li Zh, Liu H, Shi Wd, Zhang J. Chin J Integr Med. 2015 Jun;21(6):423-30. Doi: 10.1007/S11655-014-1752-3. Epub 2014 May 10. Inhibitory Effect Of Tetramethylpyrazine Preconditioning On Overload Training-Induced Myocardial Apoptosis In Rats.

Song Gh, Wang Rl, Chen Zy, Zhang B, Wang Hl, Liu Ml, Gao Jp, Yan Xy. J Physiol Biochem. 2014 Sep;70(3):761-8. Doi: 10.1007/S13105-014-0344-1. Epub 2014 Jul 30. Toxic Effects Of Sodium Fluoride On Cell Proliferation And Apoptosis Of Leydig Cells Fr...

Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W. Food Chem Toxicol. 2013 Sep;59:446-54. Doi: 10.1016/J.Fct.2013.06.032. Epub 2013 Jun 28. Protective Role Of Sodium Selenite On Histopathological Lesions, D...

Mao Z, Song Z, Li G, Lv W, Zhao X, Li B, Feng X, Chen Y. Neural Regen Res. 2013 Jan 15;8(2):133-42. Doi: 10.3969/J.Issn.1673-5374.2013.02.005. 8-Hydroxy-2-(Di-N-Propylamino)Tetralin Intervenes With Neural Cell Apoptosis Following Diffuse Axonal In...

Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.

Li X, Wang L, Wang Z. Phys Med. 2015 Jun;31(4):352-9. Doi: 10.1016/J.Ejmp.2015.02.004. Epub 2015 Feb 19. Radioprotective Activity Of Neutral Polysaccharides Isolated From The Fruiting Bodies Of Hohenbuehelia Serotina.

Wei T, Tian W, Yan H, Shao G, Xie G. Cell Mol Neurobiol. 2014 Nov;34(8):1165-73. Doi: 10.1007/S10571-014-0091-4. Epub 2014 Aug 22. Protective Effects Of Phillyrin On H2O 2-Induced Oxidative Stress And Apoptosis In Pc12 Cells.

Ding H, Wang H, Zhao Y, Sun D, Zhai X. Cell Mol Neurobiol. 2015 Jul;35(5):623-32. Doi: 10.1007/S10571-015-0156-Z. Epub 2015 Jan 18. Protective Effects Of Baicalin On A??????????????-Induced Learning And Memory Deficit, Oxidative Stress, And Apopto...

Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...

Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...

Duo J, Ying Gg, Wang Gw, Zhang L. Mol Med Rep. 2012 Jun;5(6):1453-6. Doi: 10.3892/Mmr.2012.845. Epub 2012 Mar 22. Quercetin Inhibits Human Breast Cancer Cell Proliferation And Induces Apoptosis Via Bcl-2 And Bax Regulation.

Li Ly, Zhang Lj, Jia Jy, Zhao Q, Wang Eb, Li Qw. Int J Clin Exp Pathol. 2013;6(2):212-23. Epub 2013 Jan 15. Does Dynamic Immobilization Reduce Chondrocyte Apoptosis And Disturbance To The Femoral Head Perfusion?

Ji Cf, Ji Yb. Oncol Lett. 2014 May;7(5):1728-1732. Epub 2014 Mar 7. Laminarin-Induced Apoptosis In Human Colon Cancer Lovo Cells.

Tang C, Lu Yh, Xie Jh, Wang F, Zou Jn, Yang Js, Xing Yy, Xi T. Anticancer Drugs. 2009 Apr;20(4):249-58. Doi: 10.1097/Cad.0B013E328327D476. Downregulation Of Survivin And Activation Of Caspase-3 Through The Pi3K/Akt Pathway In Ursolic Acid-Induced ...

Meng Fj, Jiao Sm, Yu B. Int J Mol Med. 2012 Aug;30(2):263-70. Doi: 10.3892/Ijmm.2012.987. Epub 2012 May 9. Picroside Ii Protects Cardiomyocytes From Hypoxia/Reoxygenation-Induced Apoptosis By Activating The Pi3K/Akt And Creb Pathways.

Tian W, Wei T, Li B, Wang Z, Zhang N, Xie G. Plos One. 2014 May 6;9(5):E96775. Doi: 10.1371/Journal.Pone.0096775. Ecollection 2014. Pathway Of Programmed Cell Death And Oxidative Stress Induced By ??-Hydroxybutyrate In Dairy Cow Abomasum Smooth Mu...

Zhang S, Zhang C, Li Y, Wang P, Yue Z, Xie S. Biomed Pharmacother. 2011 Sep;65(6):436-42. Doi: 10.1016/J.Biopha.2011.04.010. Epub 2011 Jun 12. Mir-98 Regulates Cisplatin-Induced A549 Cell Death By Inhibiting Tp53 Pathway.

Xie Sy, Li Yj, Wang Py, Jiao F, Zhang S, Zhang Wj. Oncol Rep. 2010 Jun;23(6):1693-700. Mirna-Regulated Expression Of Oncogenes And Tumor Suppressor Genes In The Cisplatin-Inhibited Growth Of K562 Cells.

Zhang D, Fu M, Song C, Wang C, Lin X, Liu Y. Neural Regen Res. 2012 Oct 25;7(30):2347-53. Doi: 10.3969/J.Issn.1673-5374.2012.30.004. Expressions Of Apoptosis-Related Proteins In Rats With Focal Cerebral Ischemia After Angong Niuhuang Sticker Point...

Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...

Dong Hy, Wang Zf. Chin J Cancer Res. 2014 Apr;26(2):200-7. Doi: 10.3978/J.Issn.1000-9604.2014.04.07. Antitumor Effects Of Artesunate On Human Breast Carcinoma Mcf-7 Cells And Igf-Ir Expression In Nude Mice Xenografts.

Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.

Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J, Lu J. Oncol Rep. 2013 Jun;29(6):2291-6. Doi: 10.3892/Or.2013.2390. Epub 2013 Apr 8. Leptin Induces Cell Proliferation And Reduces Cell Apoptosis By Activating C-Myc In Cervical Cancer.

Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.

Hou X, Zhang R, Lv H, Cai X, Xie G, Song X. Neural Regen Res. 2014 Jan 15;9(2):164-70. Doi: 10.4103/1673-5374.125345. Acupuncture At Baihui And Dazhui Reduces Brain Cell Apoptosis In Heroin Readdicts.

Lu Wn, Zheng Fp, Lai Dw, Li H. Chin J Integr Med. 2015 Apr 6. [Epub Ahead Of Print] Xuezhikang () Reduced Renal Cell Apoptosis In Streptozocin-Induced Diabetic Rats Through Regulation Of Bcl-2 Family.

Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...

Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.

Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.

Wang Tb, Wei Xq, Lin Wh, Shi Hp, Dong Wg. Clin Exp Med. 2012 Jun;12(2):89-95. Doi: 10.1007/S10238-011-0143-8. Epub 2011 Jun 10. The Inhibition Of Endostar On The Angiogenesis And Growth Of Gastrointestinal Stromal Tumor Xenograft.

Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...

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Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

Ran Yh, Wang H. J Zhejiang Univ Sci B. 2011 Oct;12(10):835-45. Doi: 10.1631/Jzus.B1100067. Iptakalim, An Atp-Sensitive Potassium Channel Opener, Confers Neuroprotection Against Cerebral Ischemia/Reperfusion Injury In Rats By Protecting Neurovascul...

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16 Customer Q&As for Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)

Question

Does MA1004 anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

You can see on the product datasheet, MA1004 anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-18

Question

We have seen staining in human testis. Are there any suggestions? Is anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) supposed to stain testis positively?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

Based on literature testis does express BCL2. Based on Uniprot.org, BCL2 is expressed in thyroid gland, testis, among other tissues. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-18

Question

We need to test anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 on human thyroid gland for research purposes, then I may be interested in using anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

The products we sell, including anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-21

Question

Would anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 work for WB with thyroid gland?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

According to the expression profile of thyroid gland, BCL2 is highly expressed in thyroid gland. So, it is likely that anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 will work for WB with thyroid gland.

Boster Scientific Support

Answered: 2019-11-08

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-31

Question

Do you have a BSA free version of anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 available?

E. Li

Verified customer

Asked: 2019-07-04

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-04

Question

I was wanting to use your anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) for WB for human thyroid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thyroid gland identification?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

As indicated on the product datasheet, MA1004 anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thyroid gland in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-24

Question

We are currently using anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-02-08

Answer

The anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) (MA1004) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-08

Question

Here is the WB image, lot number and protocol we used for thyroid gland using anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004. Please let me know if you require anything else.

F. Huang

Verified customer

Asked: 2018-09-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-24

Question

We want using your anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) for response to vitamin e studies. Has this antibody been tested with western blotting on intestinal cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-09-06

Answer

Thank you for your inquiry. This MA1004 anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) is tested on rat heart tissue, tissue lysate, spleen tissue, small intestine tissue, intestinal cancer tissue, liver tissue, mammary cancer tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-09-06

Question

My boss were satisfied with the WB result of your anti-Bcl-2 antibody (Monoclonal, Bcl-2-100). However we have been able to see positive staining in thyroid gland mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is BCL2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

From literature, thyroid gland does express BCL2. Generally BCL2 expresses in mitochondrion outer membrane. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-06-15

Question

We ordered your anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) for ICC on thyroid gland last year. I am using human, and I plan to use the antibody for IHC next. We want examining thyroid gland as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

J. Johnson

Verified customer

Asked: 2018-03-13

Answer

I viewed the website and datasheets of our anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) and I see that MA1004 has been tested on human in both ICC and IHC. Thus MA1004 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-03-13

Question

Is this MA1004 anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) reactive to the isotypes of BCL2?

K. Kulkarni

Verified customer

Asked: 2018-01-03

Answer

The immunogen of MA1004 anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) is Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-03

Question

I have a question about product MA1004, anti-Bcl-2 antibody (Monoclonal, Bcl-2-100). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-23

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1004 anti-Bcl-2 antibody (Monoclonal, Bcl-2-100), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-23

Question

Is a blocking peptide available for product anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) (MA1004)?

G. Mangal

Verified customer

Asked: 2016-08-15

Answer

We do provide the blocking peptide for product anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) (MA1004). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-08-15

Question

I see that the anti-Bcl-2 antibody (Monoclonal, Bcl-2-100) MA1004 works with WB, what is the protocol used to produce the result images on the product page?

E. Gonzalez

Verified customer

Asked: 2013-04-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-04-12

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MA1004
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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