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Product Info Summary
| SKU: | A07380 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-Bcl-B BCL2L10 Antibody
SKU/Catalog Number
A07380
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bcl-B BCL2L10 Antibody (Catalog # A07380). Tested in ELISA, WB, ICC applications. This antibody reacts with Human.
Storage & Handling
Bcl-B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bcl-B BCL2L10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07380)
Host
Rabbit
Contents
Bcl-B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bcl-B antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B. The immunogen is located within amino acids 90 - 140 of Bcl-B.
Reactive Species
A07380 is reactive to BCL2L10 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
23.2 kDa
Background of BCL2L10
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07380 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 μg/mL.
Specific Publications For Anti-Bcl-B BCL2L10 Antibody (A07380)
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