Anti-Bcl-G BCL2L14 Antibody

Product Name

Anti-Bcl-G BCL2L14 Antibody

View all BCL2L14 Antibodies

SKU/Catalog Number

A09347

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Bcl-G BCL2L14 Antibody (Catalog # A09347). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Bcl-G antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Bcl-G BCL2L14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09347)

Host

Rabbit

Contents

Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G. The immunogen is located within the first 50 amino acids of Bcl-G.

Cross-reactivity

Although antibody should react with both isoforms, only the Bcl-GS protein has been observed

Reactive Species

A09347 is reactive to BCL2L14 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

36.6 kDa

Background of BCL2L14

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09347 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BCL2L14

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