Anti-Bcr (Ab-177) Antibody

Product Name

Anti-Bcr (Ab-177) Antibody

View all BCR Antibodies

SKU/Catalog Number

A00022-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Bcr (Ab-177) Antibody (Catalog # A00022-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bcr (Ab-177) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00022-1)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Clone Number

1E12E1

Isotype

IgG

Immunogen

Peptide sequence around aa. 175~179 (P-F-Y-V-N) derived from Human Bcr.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00022-1 is reactive to BCR in Human, Mouse

Applications

A00022-1 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

142.819kDa

Background of BCR

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 130 160 210kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BCR using anti-BCR antibody (A00022-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCR antigen affinity purified polyclonal antibody (Catalog # A00022-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for BCR.

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Immunofluorescence staining of methanol-fixed Hela cells using Bcr(Ab-177) Antibody #A00022-1.

Gene/Protein Information For BCR (Source: Uniprot.org, NCBI)

Alternative Names

ALL; ALLD22S11; BCR; BCR1; breakpoint cluster region protein; breakpoint cluster region; CMLEC 2.7.11.1; D22S662; PHL; PHLFLJ16453; Renal carcinoma antigen NY-REN-26 BCR ALL1, CML, D22S11, D22S662, PHL, BCR BCR activator of RhoGEF and GTPase breakpoint cluster region protein|BCR, RhoGEF and GTPase activating protein|BCR/FGFR1 chimera protein|FGFR1/BCR chimera protein|breakpoint cluster region|renal carcinoma antigen NY-REN-26

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