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- Table of Contents
BECN1 antibody (M00327) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Immunofluorescent analysis of Hela cells, using Beclin 1 Antibody.
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Anti-Beclin 1 BECN1 Monoclonal Antibody
BECN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
BECN1 antibody was positively detected by WB in:
HEK293 cell, NIH/3T3 cell, C6 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
BECN1 antibody was positively detected by IHC in:
human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
BECN1 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Beclin 1 BECN1 Monoclonal Antibody
View all BECN1 Antibodies
View all BECN1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADA-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Beclin 1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
51.9 kDa
Cite This Product
Anti-Beclin 1 BECN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00327)
Protocols
Western Blot protocol for BECN1 antibody
Immunohistochemistry (IHC) protocol for BECN1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for BECN1 antibody
Publications
Application:
Species:
Collagen type I alpha 1 induces radio-resistance in hypoxic glioblastoma cells by promoting autophagy
Published: 2025-08-12
Journal: JOURNAL OF NEURO-ONCOLOGY
Application: WB
Impact Factor: 3.10
Species: Human
PMID: 40794247
Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability
Published: 2024-05-14
Journal: Cardiovascular Toxicology
Application: WB
Impact Factor: 3.40
Species: Human
PMID: 38743320
Liensinine sensitizes colorectal cancer cells to oxaliplatin by targeting HIF-1a to inhibit autophagy
Published: 2024-04-22
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 6.70
Species: Mouse
PMID: 38703660
Beclin1 overexpression suppresses tumor cell proliferation and survival via an autophagy‑dependent pathway in human synovial sarcoma cells
Published: 2018-07-25
Journal: ONCOLOGY REPORTS
Application: IF
Impact Factor: 2.98
Species: Human
PMID: 30066884
Review
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Product Promise
We promise Anti-Beclin 1 BECN1 Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00327
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026