Anti-Beclin-1 BECN1 Antibody

Product Name

Anti-Beclin-1 BECN1 Antibody

View all BECN1 Antibodies

SKU/Catalog Number

A00327

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Beclin-1 BECN1 Antibody (Catalog # A00327). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Beclin-1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Beclin-1 BECN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00327)

Host

Rabbit

Contents

Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Beclin-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beclin-1. The immunogen is located within the last 50 amino acids of Beclin-1.

Reactive Species

A00327 is reactive to BECN1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

51.9 kDa

Background of BECN1

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00327 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BECN1

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