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- Table of Contents
ACTB antibody (M01263-3) performs well in Flow Cytometry, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Designing a Western blot or IHC for ACTB? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Beta-Actin Antibody
ACTB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:1000
IF: 1:10-1:50
IHC-P: 1:25
FC: 1:25
IHC: 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti-Beta-Actin Antibody
View all ACTB Antibodies
View all ACTB Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
137CT26.1.1
Isotype
IgG1,Igk
Immunogen
This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
Calculated Molecular Weight
41.7 kDa
Background of ACTB
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Cite This Product
Anti-Beta-Actin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-3)
Publications
Application:
Species:
Platelet factors 4 produces an antidepressant effect in mice via inhibition of neuroinflammation
Published: 2026-04-15
Journal: Journal of Advanced Research
Application: ELISA
Impact Factor: 13.00
Species: Mouse
PMID: 41990973
Investigating the Antifibrotic Action of Foeniculum vulgare Root Bark Volatile Oil Through the HK2/PKM2/LDHA Pathway
Published: 2025-09-16
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 6.30
Species: Mouse
PMID: 40956586
ADAM17 Aggravates the Inflammatory Response by Modulating Microglia Polarization Through the TGF-β1/Smad Pathway Following Experimental Traumatic Brain Injury
Published: 2023-06-27
Journal: JOURNAL OF NEUROTRAUMA
Application: WB
Impact Factor: 4.20
Species: Rat,Mouse
PMID: 37029898
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Product Promise
We promise Anti-Beta-Actin Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01263-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 30, 2026