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Product Info Summary
| SKU: | M01263-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-Beta-Actin Antibody
SKU/Catalog Number
M01263-3
Size
80 µl
Description
Boster Bio Anti-Beta-Actin Antibody (Catalog # M01263-3). Tested in WB, Flow Cytometry, IF, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Beta-Actin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-3)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
137CT26.1.1
Isotype
IgG1,Igk
Immunogen
This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
Reactive Species
M01263-3 is reactive to ACTB in Human, Mouse, Rat
Calculated molecular weight
41.7 kDa
Background of ACTB
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01263-3 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IF: 1:10-1:50
IHC-P: 1:25
FC: 1:25
IHC: 1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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All lanes : Anti-ACTB Antibody at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: C2C12 whole cell lysate
Lane 3: C6 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: MCF-7 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 42 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-ACTB Antibody in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35g/lane). ACTB (arrow) was detected using the purified Mab.
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All lanes : Anti-ACTB Antibody at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: NIH-3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 42 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M01263-3 staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded H.skeletal muscle section using Beta-Actin Antibody. M01263-3 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody. M01263-3 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Specific Publications For Anti-Beta-Actin Antibody (M01263-3)
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