Anti-Beta-Actin Actb Antibody (Monoclonal, 31A06)

Product Name

Anti-Beta-Actin Actb Antibody (Monoclonal, 31A06)

View all Actb Antibodies

SKU/Catalog Number

M01263-9

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Beta-Actin Actb Antibody (Monoclonal, 31A06) catalog # M01263-9. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beta-Actin Actb Antibody (Monoclonal, 31A06) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-9)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

31A06

Isotype

Mouse IgG1

Immunogen

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M01263-9 is reactive to Actb in Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat

Calculated molecular weight

41.7 kDa

Background of Actb

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01263-9 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.05-0.1μg/ml, Human, mouse, rat, pig, monkey, rabbit, chicken
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information Actb

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