Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)

beta-Actin antibody

Boster Bio Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) catalog # MA1115. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1115
Size: 100 μg/vial
Reactive Species: Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)

View all beta-Actin Antibodies

SKU/Catalog Number

MA1115

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) catalog # MA1115. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1115)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

AC-15

Isotype

Mouse IgG1

Immunogen

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1115 is reactive to Actb in Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat

Applications

MA1115 is guaranteed for IHC, WB Boster Guarantee

Background of beta-Actin

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.05-0.1μg/ml, Human, mouse, rat, pig, monkey, rabbit, chicken
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For Actb (Source: Uniprot.org, NCBI)

Uniprot ID

P60711

Gene ID

81822

Gene Name

Actb

Full Name

Actin, cytoplasmic 1

Weight

41.737kDa

Superfamily

actin family

Alternative Names

ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 Actb|Actx|actin, beta|actin, cytoplasmic 1|beta-actin|cytoplasmic beta-actin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Actb, check out the Actb Infographic

Actb infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Actb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

MA1115 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas

Rat bone mesenchymal stem cells exert antiproliferative effects on nicotine‑exposed T cells via iNOS production

Oxyresveratrol Inhibits IL-1β-Induced Inflammation via Suppressing AKT and ERK1/2 Activation in Human Microglia, HMC3

Artocarpus lakoocha Extract Inhibits LPS-Induced Inflammatory Response in RAW 264.7 Macrophage Cells

Kaempferia parviflora Extract Inhibits STAT3 Activation and Interleukin-6 Production in HeLa Cervical Cancer Cells

Inhibiting FAK–Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells

Ischemic postconditioning reduced myocardial ischemia-reperfusion injury: The roles of melatonin and uncoupling protein 3

Boesenbergia rotunda extract accelerates human keratinocyte proliferation through activating ERK1/2 and PI3K/Akt kinases

Physiological and pharmacological effects of melatonin on remote ischemic perconditioning after myocardial ischemia‐reperfusion injury in rats: Role of Cybb, Fas, NfκB, Irisin signaling pathway
Species: Rat

Liu ZZ,Han ZD,Liang YK,Chen JX,Wan S,Zhuo YJ,Cai ZD,Deng YL,Lin ZY,Mo RJ,He HC,Zhong WD.TRIB1 induces macrophages to M2 phenotype by inhibiting IKB-zeta in prostate cancer.Cell Signal.2019 Jul;59:152-162.doi:10.1016/j.cellsig.2019.03.017.Epub 2019 Mar 26.PMID:30926388.
Species: Human,Mouse
MA1115 usage in article: APP:WB, SAMPLE:PC3 CELL,DU145 CELL AND THP-1 CELL, DILUTION:1:3000

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7 Customer Q&As for Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)

Question

Is a blocking peptide available for product anti-Beta-Actin antibody (Monoclonal, AC-15) (MA1115)?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

We do provide the blocking peptide for product anti-Beta-Actin antibody (Monoclonal, AC-15) (MA1115). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-25

Question

We are currently using anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

The anti-Beta-Actin antibody (Monoclonal, AC-15) (MA1115) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-19

Question

I see that the anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-03

Question

Is this MA1115 anti-Beta-Actin antibody (Monoclonal, AC-15) reactive to the isotypes of ACTB?

Verified Customer

Verified customer

Asked: 2019-01-07

Answer

The immunogen of MA1115 anti-Beta-Actin antibody (Monoclonal, AC-15) is slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-07

Question

I have attached the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-10-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-30

Question

Will anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 work on feline IHC with b-cell lymphoma?

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

Our lab technicians have not tested anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline b-cell lymphoma in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-12

Question

I was wanting to use your anti-Beta-Actin antibody (Monoclonal, AC-15) for IHC for human cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cajal-retzius cell fetal brain cortex identification?

Verified Customer

Verified customer

Asked: 2017-08-24

Answer

It shows on the product datasheet, MA1115 anti-Beta-Actin antibody (Monoclonal, AC-15) has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-24

Size

Conjugation

Total: $315

SKU:MA1115

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MA1115
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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