SKU MA1115
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number AC-15
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB


Product Name Anti-Beta-Actin Antibody (Monoclonal, AC-15)
SKU/Catalog Number MA1115
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-Beta-Actin Antibody (Monoclonal, AC-15) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1115)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat
Western blot, 0.25-0.5μg/mlμg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1115 1 WB anti beta actin antibody monoclonal ac 15.jpg

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: Rat Cardiac Muscle Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: A453 Cell Lysate
Lane 6: HE

/antibody/ma1115 2 WB anti beta actin antibody monoclonal ac 15.jpg

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: SMMC Cell

/antibody/ma1115 3 IHC anti beta actin antibody monoclonal ac 15.jpg

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Mouse Intestine Tissue

/antibody/ma1115 4 IHC anti beta actin antibody monoclonal ac 15.jpg

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Rat Intestine Tissue

/antibody/ma1115 5 IHC anti beta actin antibody monoclonal ac 15.jpg

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/antibody/ma1115 6 IHC anti beta actin antibody monoclonal ac 15.jpg

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Human Lung Cancer Tissue

/antibody/ma1115 7 IHC anti beta actin antibody monoclonal ac 15.jpg

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source:

Uniprot Id P60711
Gene Name ACTB
Protein Name Actin, cytoplasmic 1
Alternative Names Actin, cytoplasmic 1;Beta-actin;Actin, cytoplasmic 1, N-terminally processed;Actb;
Subcellular Localization Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Molecular Weight 41737 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.


Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Areas Actin, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction

*You can search these to find other products in these research areas.
Background The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

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Order Product (MA1115)


Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships next business day.


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The Effects of Aquaporin-1 in Pulmonary Edema Induced by Fat Embolism Syndrome
Slit2?Robo1 signaling promotes the adhesion, invasion and migration of tongue?carcinoma?cellsvia upregulating matrix metalloproteinases 2 and 9, and downregulating E?cadherin
Downregulation of HP1? suppresses proliferation of cholangiocarcinoma by restoring SFRP1 expression
MicroRNA-21a-5p Functions on the Regulation of Melanogenesis by Targeting Sox5 in Mouse Skin Melanocytes
The ORF3 Protein of Genotype 1 Hepatitis E Virus Suppresses TLR3-induced NF-?B Signaling via TRADD and RIP1
Effects of dexmedetomidine postconditioning on myocardial ischemia and the role of the PI3K/Akt-dependent signaling pathway in reperfusion injury
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Metformin Prevents Dopaminergic Neuron Death in MPTP/P-Induced Mouse Model of Parkinson's Disease via Autophagy and Mitochondrial ROS Clearance
Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS
Selective CDK7 inhibition with BS-181 suppresses cell proliferation and induces cell cycle arrest and apoptosis in gastric cancer
Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells
Expression and Distribution of the Guanine Nucleotide-binding Protein Subunit Alpha-s in Mice Skin Tissues and Its Association with White and Black Coat Colors
miR-223 reverses the resistance of EGFR-TKIs through IGF1R/PI3K/Akt signaling pathway
SENP?1 enhances hypoxia?induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia?inducible factor?1?
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway
Vagus nerve stimulation attenuates myocardial ischemia/reperfusion injury by inhibiting the expression of interleukin-17A
MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes
Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
The Expression of Porcine Prdx6 Gene Is Up-Regulated by C/EBP? and CREB
Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro
Traditional Chinese medicine Qili qiangxin inhibits cardiomyocyte apoptosis in rats following myocardial infarction
The Protective Role of Interleukin-33 in Myocardial Ischemia and Reperfusion Is Associated with Decreased HMGB1 Expression and Up-Regulation of the P38 MAPK Signaling Pathway
High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue
Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats
Experimental study of the protective effects of SYVN1 against diabetic retinopathy
Lithium chloride attenuates the abnormal osteogenic/adipogenic differentiation of bone marrow-derived mesenchymal stem cells obtained from rats with steroid-related osteonecrosis by activating the ?-catenin pathway
Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway
Autologous bone marrow mononuclear cell transplantation delays progression of carotid atherosclerosis in rabbits
Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-?B
Rhizoma Paridis Saponins Suppresses Tumor Growth in a Rat Model of N-Nitrosomethylbenzylamine-Induced Esophageal Cancer by Inhibiting Cyclooxygenases-2 Pathway
MiR-338-3p inhibits epithelial-mesenchymal transition in gastric cancer cells by targeting ZEB2 and MACC1/Met/Akt signaling
Fructus phyllanthi tannin fraction induces apoptosis and inhibits migration and invasion of human lung squamous carcinoma cells in vitro via MAPK/MMP pathways
Evaluating the Role of PTH in Promotion of Chondrosarcoma Cell Proliferation and Invasion by Inhibiting Primary Cilia Expression
Diagnostic and prognostic value of a disintegrin and metalloproteinase-17 in patients with gliomas
Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
Apelin-13 Decreases Lipid Storage in Hypertrophic Adipocytes?In Vitro?Through the Upregulation of AQP7 Expression by the PI3K Signaling Pathway
Preparation and Characterization of a Novel Aspirin Derivative with Anti-Thrombotic and Gastric Mucosal Protection Properties
Powerful inner/outer controlled multi-target magnetic nanoparticle drug carrier prepared by liquid photo-immobilization
Mest Attenuates CCl4-Induced Liver Fibrosis in Rats by Inhibiting the Wnt/?-Catenin Signaling Pathway
The effects of aspirin plus cisplatin on SGC7901/CDDP cells?in vitro
Anti-fibrosis effects of Huisheng oral solution in CCl4-induced hepatic fibrosis in rat
GGPPS1 predicts the biological character of hepatocellular carcinoma in patients with cirrhosis
Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis
ERK/CANP rapid signaling mediates 17?-estradiol-induced proliferation of human breast cancer cell line MCF-7 cells
N-n-Butyl Haloperidol Iodide Ameliorates Cardiomyocytes Hypoxia/Reoxygenation Injury by Extracellular Calcium-Dependent and -Independent Mechanisms
VX680 suppresses the growth of HepG2 cells and enhances the chemosensitivity to cisplatin
Hypoxia/oxidative stress alters the pharmacokinetics of CPU86017-RS through mitochondrial dysfunction and NADPH oxidase activation
c?Ski activates cancer?associated fibroblasts to regulate breast cancer cell invasion
Electrospun Poly(L-lactide)/Poly(?-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells
Histone acetyltransferase PCAF Up-regulated cell apoptosis in hepatocellular carcinoma via acetylating histone H4 and inactivating AKT signaling
Sox2 enhances the tumorigenicity and chemoresistance of cancer stem-like cells derived from gastric cancer
Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation
Combination Therapy with Losartan and Pioglitazone Additively Reduces Renal Oxidative and Nitrative Stress Induced by Chronic High Fat, Sucrose, and Sodium Intake
Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs
Down-Regulation of AP-4 Inhibits Proliferation, Induces Cell Cycle Arrest and Promotes Apoptosis in Human Gastric Cancer Cells
CPU86017-RS attenuate hypoxia-induced testicular dysfunction in mice by normalizing androgen biosynthesis genes and pro-inflammatory cytokines
Cellular pharmacokinetic mechanisms of adriamycin resistance and its modulation by 20(S)-ginsenoside Rh2 in MCF-7/Adr cells
Insulin therapy stimulates lipid synthesis and improves endocrine functions of adipocytes in dietary obese C57BL/6 mice
HSP70: a promising target for laryngeal carcinoma radiaotherapy by inhibiting cleavage and degradation of nucleolin
Urocortin promotes the development of vasculitis in a rat model of thromboangiitis obliterans via corticotrophin-releasing factor type 1 receptors
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to beta-actin antibody, actb antibody, actin beta antibody