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ACTB antibody (M01263-5) performs well in WB and shows reactivity with Goat, Human, Monkey, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 47 Publications

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m01263 5 actb primary antibodies wb testing 1

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Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.

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Anti-beta Actin Mouse Monoclonal Antibody

ACTB antibody

47 Publications

Reactive Species	Host	Application
Goat, Human, Monkey, Mouse, Rat	Mouse	WB

Tested Applications

ACTB antibody was positively detected by WB in:

Hela, Human fetal kidney, 3T3 cell, PC-12 cell, COS-1 cell, Goat muscle

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

Human

Tested

Goat

Tested

Product Information

Product Name

Anti-beta Actin Mouse Monoclonal Antibody
View all ACTB Antibodies

Form

Liquid

Uniprot

P60709

Gene ID

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Mouse

Contents

Mouse IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

17A11

Isotype

IgG2b

Immunogen

A synthesized peptide derived from human beta Actin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

42 kDa

Calculated Molecular Weight

41.7 kDa

Cite This Product

Anti-beta Actin Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-5)

Protocols

Western Blot protocol for ACTB antibody

Review Protocol

Publications

Proprotein Convertase Subtilisin/Kexin Type 9 Induces Platelet‐Derived Transforming Growth Factor‐β to Promote Myocardial Fibrosis After Myocardial Infarction

Published: 2026-01-21

Journal: HUMAN MUTATION

Application: WB

Impact Factor: 3.70

Species: Mouse

PMID: 41573336

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Free Secondary Antibody

Anti-beta Actin Mouse Monoclonal Antibody

ACTB antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Proprotein Convertase Subtilisin/Kexin Type 9 Induces Platelet‐Derived Transforming Growth Factor‐β to Promote Myocardial Fibrosis After Myocardial Infarction

Albumin antagonizes Alzheimer’s disease-related Tau pathology and enhances cognitive performance by inhibiting aberrant Tau aggregation

miR-276 and miR-182013-5p modulate insect metamorphosis and reproduction via dually regulating juvenile hormone acid methyltransferase

MiR-146a-5p-enriched exosomes inhibit M1 macrophage activation and inflammatory response by targeting CD80

Fibroblast growth factor 21 improves diabetic cardiomyopathy by inhibiting ferroptosis via ferritin pathway

Pulmonary artery smooth muscle cell pyroptosis promotes the proliferation of PASMCs by paracrine IL‑1β and IL‑18 in monocrotaline‑induced pulmonary arterial hypertensive rats

Moxibustion ameliorates cerebral ischemia–reperfusion injury by regulating ferroptosis in rats

Transcriptomic and proteomic analyses provide insights into the adaptive responses to the combined impact of salinity and alkalinity in Gymnocypris przewalskii

Central administration of human opiorphin alleviates dextran sodium sulfate-induced colitis in mice through activation of the endogenous opioid system

PERK reprograms hematopoietic progenitor cells to direct tumor-promoting myelopoiesis in the spleen

Perinatal Combinational Exposure to Bisphenol A and a High-Fat Diet Contributes to Transgenerational Dysregulation of Cardiovascular and Metabolic Systems in Mice

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis

Effects of L-theanine on intestinal morphology, barrier function, and MAPK signaling pathways in diquat-challenged piglets

Curcumin suppresses neuroinflammation to protect neurons by preventing NLRP3 inflammasome activation

NF-κB–Dependent Snail Expression Promotes Epithelial–Mesenchymal Transition in Mastitis

Rubus chingii var. suavissimus alleviates high-fat diet-induced lipid metabolism disorder via modulation of the PPARs/SREBP pathway in Syrian golden hamsters

Protective effect of surfactin on copper sulfate-induced inflammation, oxidative stress, and hepatic injury in zebrafish

l-Borneol ameliorates cerebral ischaemia by downregulating the mitochondrial calcium uniporter-induced apoptosis cascade in pMCAO rats

All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells

Forsythoside A protects against lipopolysaccharide-induced acute lung injury through up-regulating microRNA-124

The protective effect and mechanism of pedunculoside on DSS (dextran sulfate sodium) induced ulcerative colitis in mice

Ac2-26 Alleviates Brain Injury after Cardiac Arrest and Cardiopulmonary Resuscitation in Rats via the eNOS Pathway

Neuroprotective Effects of Celastrol on Transient Global Cerebral Ischemia Rats via Regulating HMGB1/NF-κB Signaling Pathway

Tilapia Skin Peptides Ameliorate Diabetic Nephropathy in STZ-Induced Diabetic Rats and HG-Induced GMCs by Improving Mitochondrial Dysfunction

Baicalein Alleviates Erectile Dysfunction Associated with Streptozotocin-Induced Type I Diabetes by Ameliorating Endothelial Nitric Oxide Synthase Dysfunction, Inhibiting Oxidative Stress and Fibrosis

Decoy receptor 2 mediation of the senescent phenotype of tubular cells by interacting with peroxiredoxin 1 presents a novel mechanism of renal fibrosis in diabetic nephropathy

Overexpression and biological function of PRDX6 in human cervical cancer

Nuclear import of NLS- RARα is mediated by importin α/β

High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1

Adipogenesis in ducks interfered by small interfering ribonucleic acids of peroxisome proliferator-activated receptor γ gene

NLS-RARα contributes to differentiation block and increased leukemogenic potential in vivo

miR‑483‑3p regulates osteogenic differentiation of bone marrow mesenchymal stem cells by targeting STAT1

Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle, Pelodiscus sinensis

Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4

Codon optimization of G protein enhances rabies virus-induced humoral immunity

Ac-YVAD-cmk improves neurological function by inhibiting caspase-1-mediated inflammatory response in the intracerebral hemorrhage of rats

Overexpression of TG2 enhances the differentiation of ectomesenchymal stem cells into neuron‑like cells and promotes functional recovery in adult rats following spinal cord injury

Role of neutrophil extracellular traps in chronic kidney injury induced by bisphenol-A

The development of tertiary amine cationic lipids for safe and efficient siRNA delivery

Protective Effects of Inorganic and Organic Selenium on Heat Stress in Bovine Mammary Epithelial Cells

Cdh1 overexpression improves emotion and cognitive-related behaviors via regulating hippocampal neuroplasticity in global cerebral ischemia rats

Astragalus saponins inhibit cell growth, aerobic glycolysis and attenuate the inflammatory response in a DSS-induced colitis model

Polydatin attenuates diet-induced nonalcoholic steatohepatitis and fibrosis in mice

7B2 chaperone knockout in APP model mice results in reduced plaque burden

Against NF-κB/thymic stromal lymphopoietin signaling pathway, catechin alleviates the inflammation in allergic rhinitis

6-Gingerol Ameliorates Behavioral Changes and Atherosclerotic Lesions in ApoE−/− Mice Exposed to Chronic Mild Stress

Sodium butyrate alleviates LPS-induced acute lung injury in mice via inhibiting HMGB1 release

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