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- Table of Contents
TUBB antibody (M05613-6) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-beta Tubulin Rabbit Monoclonal Antibody
TUBB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
TUBB antibody was positively detected by WB in:
human Hela whole cell, human K562 whole cell, human MCF-7 whole cell, human A549 whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TUBB antibody was positively detected by IHC in:
Rat stomach tissue, Human Hodgkin's lymphoma tissue, Human astrocytoma tissue, Mouse skeletal muscle - gastrocnemius tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:1000-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-beta Tubulin Rabbit Monoclonal Antibody
View all TUBB Antibodies
View all TUBB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
17T15
Isotype
IgG
Immunogen
A synthesized peptide derived from human beta Tubulin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
49.7 kDa
Cite This Product
Anti-beta Tubulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05613-6)
Protocols
Western Blot protocol for TUBB antibody
Immunohistochemistry (IHC) protocol for TUBB antibody
Publications
Application:
Species:
Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling
Published: 2024-10-11
Journal: Cell Communication and Signaling
Application: WB
Impact Factor: 8.20
Species: Rat
PMID: 39394127
CCR7/DUSP1 signaling Axis mediates iCAF to regulates head and neck squamous cell carcinoma growth
Published: 2024-07-25
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 39067836
FBL Promotes LPS-Induced Neuroinflammation by Activating the NF-κB Signaling Pathway
Published: 2024-04-11
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.20
Species: Mouse
PMID: 38623466
Carboxy-terminus Hsc70 interacting protein exerts a tumor inhibition function in head and neck cancer
Published: 2017-07-17
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 2.66
Species: Human
PMID: 28731191
Ezetimibe-Mediated Protection of Vascular Smooth Muscle Cells from Cholesterol Accumulation through the Regulation of Lipid Metabolism-Related Gene Expression
Published: 2014-11-11
Journal: PHARMACOLOGY
Application: WB
Impact Factor: 1.58
Species: Rat
PMID: 25402258
Histamine Upregulates Nav1.8 Expression in Primary Afferent Neurons via H2 Receptors: Involvement in Neuropathic Pain
Published: 2014-07-03
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 3.78
Species: Rat
PMID: 24990156
Proteomics Analysis of Differential Expression of Chicken Brain Tissue Proteins in Response to the Neurovirulent H5N1 Avian Influenza Virus Infection
Published: 2010-06-30
Journal: JOURNAL OF PROTEOME RESEARCH
Application: WB
Impact Factor: 5.13
Species: Chicken
PMID: 20438121
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Product Promise
We promise Anti-beta Tubulin Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M05613-6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026