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Product Info Summary
| SKU: | A10479-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BFAR Antibody
SKU/Catalog Number
A10479-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BFAR Antibody (Catalog # A10479-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BFAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BFAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10479-1)
Host
Rabbit
Contents
BFAR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
BFAR antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BFAR. The immunogen is located within amino acids 300 - 350 of BFAR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is specific for RKHD3 and will not recognize the other RKHD3 family of proteins.
Reactive Species
A10479-1 is reactive to BFAR in Human, Mouse, Rat
Applications
A10479-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.738kDa
Background of BFAR
The bifunctional apoptosis inhibitor (BFAR) is scaffold protein that integrates signaling components of the cells apoptosis-regulatory machinery. BFAR is a multidomain protein capable of inhibiting apoptosis induced by TNF-family death receptors ('extrinsic pathway') as well as mitochondria-dependent apoptosis ('intrinsic pathway'). Interaction of BFAR with Bcl-2 or Bcl-XL via a SAM domain may contribute to the anti-apoptotic properties of BFAR. In addition, BFAR contains a DED-like domain that is capable of suppressing apoptosis mediated at the receptor level. BFAR is also thought to be involved in the regulation of neuronal survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BFAR antibody can be used for detection of BFAR by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of BFAR in mouse kidney tissue with BFAR antibody at 5 μg/mL.
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Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BFAR (Source: Uniprot.org, NCBI)
Gene Name
BFAR
Full Name
Bifunctional apoptosis regulator
Weight
52.738kDa
Alternative Names
BARRNF47RING finger protein 47; bifunctional apoptosis inhibitor; bifunctional apoptosis regulator BFAR BAR, RNF47 bifunctional apoptosis regulator bifunctional apoptosis regulator|RING finger protein 47|bifunctional apoptosis inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BFAR, check out the BFAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BFAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BFAR Antibody (A10479-1)
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No publications found for A10479-1
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Customer Reviews
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