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Product Info Summary
| SKU: | M06712 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-BHMT Monoclonal Antibody
SKU/Catalog Number
M06712
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BHMT Monoclonal Antibody catalog # M06712. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BHMT Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06712)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8C11H5
Isotype
IgG
Immunogen
Purified recombinant fragment of BHMT expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06712 is reactive to BHMT in Human
Applications
M06712 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.998kDa
Background of BHMT
Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of BHMT using Anti-BHMT Monoclonal Antibody (M06712).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver Tissues with AEC staining using BHMT Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-BHMT Monoclonal Antibody M06712
Western Blot (WB) analysis using BHMT Monoclonal Antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY BHMT cDNA (2).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For BHMT (Source: Uniprot.org, NCBI)
Gene Name
BHMT
Full Name
Betaine--homocysteine S-methyltransferase 1
Weight
44.998kDa
Alternative Names
betaine homocysteine methyltransferase; betaine-homocysteine methyltransferase; betaine--homocysteine S-methyltransferase 1; betaine--homocysteine S-methyltransferase; BHMT1; EC 2.1.1; EC 2.1.1.5 BHMT BHMT1, HEL-S-61p betaine--homocysteine S-methyltransferase betaine--homocysteine S-methyltransferase 1|epididymis secretory sperm binding protein Li 61p
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BHMT, check out the BHMT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BHMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BHMT Monoclonal Antibody (M06712)
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No publications found for M06712
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Recommended Resources
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Customer Reviews
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