Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 2 publication(s).
Anti-GCLC Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
PAK4 is part of the group B family of p21-activated kinases (PAK). It's known as an effector protein for cell division cycle 42(Cdc42) and protein that plays an important role in regulating cytoskeletal organization and cell morphology. PAK4 expression is eminent in many cancer cell lines, and is involved in tumorigenesis. PAK proteins are significant effectors that associate Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins are targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 cooperates specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and is involved in the reorganization of the actin cytoskeleton. PAK4 activates the JNK pathway. PAK4 phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments.
|Product Name||Anti-GCLC Antibody
See all GCLC primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for GCLC detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GCLC information: Molecular Weight: 72766 MW; .|
|Cite This Product||Anti-GCLC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1492)|
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|Anti-GCLC Antibody may replace the following items: sc 492|sc 7382|sc 783|sc 56018|sc 65392|sc 130308|sc 130307|sc 56015|sc 509|sc 81002|sc 70411|sc 23960|sc 492 G|sc 578.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC(7-24aa GSPLSWEETKRHADHVRR), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For GCLC (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2729|
|Species Of This Entry||Human|
|Protein Name||Glutamate--cysteine ligase catalytic subunit|
|Superfamily||glutamate--cysteine ligase type 3 family|
|Alternative Names||GCLC|EC 220.127.116.11; gamma-ECS; Gamma-glutamylcysteine synthetase; GCS heavy chain; GCS; GLCL; GLCLCGCL; glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit|
|Post Tranlational Modifications||Phosphorylation; Reduction; Oxidation; Cleavage; Glutathionylation; Sumoylation; Methylation; Myristoylation|
|Gene Location||6p12.1, on Chromasome 6, gene sequence: NC_000006.12 (53497341..53545101, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For GCLC (Source: Uniprot.org, NCBI)
|Research Areas||Cell Biology; Lipid and Metabolism|
|Related Diseases||Malignant Neoplasms; Liver Carcinoma; Hepatotoxicity; Liver Diseases; Inflammation; Injury To Liver; Neoplasms; Diabetes Mellitus; Infarction; Lung Diseases; Steatohepatitis; Adenocarcinoma; Inflammatory Response; Malignant Neoplasm Of Lung; Cardiovascular Diseases; Schizophrenia; Fatty Liver; Fibrosis; Steatosis|
|Related Pathways||Cell Death; Pathogenesis; Response To Oxidative Stress; Cell Proliferation; Aging; Conjugation; Inflammatory Response; Cell Cycle; Localization; Transport; Translation; Neuroprotection; Cellular Homeostasis; Transsulfuration; Proestrus; Gastric Emptying; Excretion; Immune Response; Proteolysis; Cell Cycle Arrest|
|Background||GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.|
|Scientific References||PMID: 1350904 by Gipp J.J., et al. Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.|
PMID: 7726839 by Mulcahy R.T., et al. Identification of a putative antioxidant response element in the 5'- flanking region of the human gamma-glutamylcysteine synthetase heavy subunit gene.
Our Boster Quality Guarantee for Anti-GCLC Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
IHC(P): Human Rectal Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 2
|Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling||PubMed ID 26339340|
|Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway||PubMed ID 27630691|
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Note: sample size takes longer to process, average 5-7 days.
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