Anti-GCLC Antibody

GCLC antibody

Boster Bio Anti-GCLC Antibody catalog # PA1492. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: PA1492
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-GCLC Antibody

View all GCLC Antibodies

SKU/Catalog Number

PA1492

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GCLC Antibody catalog # PA1492. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GCLC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1492)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1492 is reactive to GCLC in Human, Mouse, Rat

Applications

PA1492 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

72.766kDa

Background of GCLC

GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For GCLC (Source: Uniprot.org, NCBI)

Gene Name

GCLC

Full Name

Glutamate--cysteine ligase catalytic subunit

Weight

72.766kDa

Superfamily

glutamate--cysteine ligase type 3 family

Alternative Names

EC 6.3.2.2; gamma-ECS; Gamma-glutamylcysteine synthetase; GCS heavy chain; GCS; GLCL; GLCLCGCL; glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit GCLC GCL, GCS, GLCL, GLCLC glutamate-cysteine ligase catalytic subunit glutamate--cysteine ligase catalytic subunit|GCS heavy chain|gamma-ECS|gamma-glutamylcysteine synthetase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GCLC, check out the GCLC Infographic

GCLC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GCLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1492 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Aldosterone Induces DNA Damage and Activation of Nrf2 Mainly in Tubuli of Mouse Kidneys

Zhilei Mao,Shushu Li,Lina Zhang,Mengmeng Yao,Zhu Zhou,Minjian Chen,"The mTOR/GCLc/GSH Pathway Mediates the Dose-Dependent Bidirectional Regulation of ROS Induced by TiO2 NPs in Neurogenic Cells",Oxidative Medicine and Cellular Longevity,vol.2019,Article ID 7621561,14 pages,2019.https://doi.org/10.1155/2019/7621561
Species: Human
PA1492 usage in article: APP:WB, SAMPLE:SH-SY5Y CELL, DILUTION:NA

Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway

Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling

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Order DetailsPrice
PA1492

100μg

$370
PA1492-10ug

10μg sample (liquid)

$99
PA1492-Biotin

100 μg Biotin conjugated

$570
PA1492-Cy3

100 μg Cy3 conjugated

$570
PA1492-Dylight488

100 μg Dylight488 conjugated

$570
PA1492-Dylight550

100 μg Dylight550 conjugated

$570
PA1492-Dylight594

100 μg Dylight594 conjugated

$570
PA1492-FITC

100 μg FITC conjugated

$570
PA1492-HRP

100 μg HRP conjugated

$570
PA1492-APC

100 μg APC conjugated

$670
PA1492-PE

100 μg PE conjugated

$670
PA1492-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1492
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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