Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4)

Bax inhibitor 1 antibody

Boster Bio Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4) (Catalog# M05462). Tested in WB application(s). This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M05462
Size: 100 µg
Reactive Species: Human
Host: Mouse
Application: WB

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Product Name

Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4)

View all Bax inhibitor 1 Antibodies

SKU/Catalog Number

M05462

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4) (Catalog# M05462). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05462)

Host

Mouse

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

ABM20E4

Isotype

IgG2a Kappa

Immunogen

A partial length recombinant protein of human Bi-1 (amino acids 1-200)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M05462 is reactive to TMBIM6 in Human

Applications

M05462 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

26.538kDa

Background of Bax inhibitor 1

This monoclonal antibody recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 4-6 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For TMBIM6 (Source: Uniprot.org, NCBI)

Gene Name

TMBIM6

Full Name

Bax inhibitor 1

Weight

26.538kDa

Superfamily

BI1 family

Alternative Names

BAXI1; BI1; BI-1Transmembrane BAX inhibitor motif-containing protein 6; TEGTbax inhibitor 1; testis enhanced gene transcript; Testis-enhanced gene transcript protein; transmembrane BAX inhibitor motif containing 6 TMBIM6 BAXI1, BI-1, TEGT transmembrane BAX inhibitor motif containing 6 bax inhibitor 1|testis enhanced gene transcript|testis-enhanced gene transcript protein|transmembrane BAX inhibitor motif-containing protein 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TMBIM6, check out the TMBIM6 Infographic

TMBIM6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TMBIM6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M05462 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Ly, Zhang Lj, Jia Jy, Zhao Q, Wang Eb, Li Qw. Int J Clin Exp Pathol. 2013;6(2):212-23. Epub 2013 Jan 15. Does Dynamic Immobilization Reduce Chondrocyte Apoptosis And Disturbance To The Femoral Head Perfusion?

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1 Customer Q&As for Anti-Bi-1 TMBIM6 Antibody (Monoclonal, ABM20E4)

Question

We are currently using anti-Bi-1 antibody (Monoclonal, ABM20E4) M05462 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

C. Kulkarni

Verified customer

Asked: 2015-01-08

Answer

The anti-Bi-1 antibody (Monoclonal, ABM20E4) (M05462) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-01-08

Order DetailsPrice
M05462-100ug

100 μg

$366

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$366.00

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