Anti-Bif SH3GLB1 Antibody

Product Name

Anti-Bif SH3GLB1 Antibody

View all SH3GLB1 Antibodies

SKU/Catalog Number

A04792

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Bif SH3GLB1 Antibody (Catalog # A04792). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Bif antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Bif SH3GLB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04792)

Host

Rabbit

Contents

Bif Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BIF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BIF. The immunogen is located within the last 50 amino acids of Bif.

Reactive Species

A04792 is reactive to SH3GLB1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

40.8 kDa

Background of SH3GLB1

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04792 is guaranteed for ELISA, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BIF antibody can be used for the detection of BIF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SH3GLB1

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