Anti-Biglycan Monoclonal Antibody

Biglycan antibody

Boster Bio Anti-Biglycan Monoclonal Antibody catalog # M02090. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M02090
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, WB

Product Name

Anti-Biglycan Monoclonal Antibody

View all Biglycan Antibodies

SKU/Catalog Number

M02090

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Biglycan Monoclonal Antibody catalog # M02090. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Biglycan Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02090)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEIB-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Biglycan May be involved in collagen fiber assembly.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02090 is reactive to BGN in Human, Mouse, Rat

Applications

M02090 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

41.654kDa

Background of Biglycan

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For BGN (Source: Uniprot.org, NCBI)

Gene Name

BGN

Full Name

Biglycan

Weight

41.654kDa

Superfamily

small leucine-rich proteoglycan (SLRP) family

Alternative Names

BGN; Biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A BGN DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A biglycan biglycan|bone/cartilage proteoglycan-I|dermatan sulphate proteoglycan I|small leucine-rich protein 1A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BGN, check out the BGN Infographic

BGN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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8 Customer Q&As for Anti-Biglycan Monoclonal Antibody

Question

I am interested in to test anti-Biglycan Monoclonal antibody M02090 on rat brain for research purposes, then I may be interested in using anti-Biglycan Monoclonal antibody M02090 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

The products we sell, including anti-Biglycan Monoclonal antibody M02090, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-16

Question

What sample was tested in WB and IHC applications with M02090?

Verified customer

Asked: 2020-01-09

Answer

Western blot analysis of Biglycan expression in C2C12 cell lysate.

Boster Scientific Support

Answered: 2020-01-09

Question

We are currently using anti-Biglycan Monoclonal antibody M02090 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

The anti-Biglycan Monoclonal antibody (M02090) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-22

Question

I see that the anti-Biglycan Monoclonal antibody M02090 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-17

Question

I was wanting to use your anti-Biglycan Monoclonal antibody for IP for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

As indicated on the product datasheet, M02090 anti-Biglycan Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-09

Question

Will anti-Biglycan Monoclonal antibody M02090 work on canine WB with bone?

L. Mangal

Verified customer

Asked: 2017-04-10

Answer

Our lab technicians have not validated anti-Biglycan Monoclonal antibody M02090 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Biglycan Monoclonal antibody M02090 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine bone in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-04-10

Question

Will anti-Biglycan Monoclonal antibody M02090 work for IP with brain?

O. Dhar

Verified customer

Asked: 2015-05-20

Answer

According to the expression profile of brain, BGN is highly expressed in brain. So, it is likely that anti-Biglycan Monoclonal antibody M02090 will work for IP with brain.

Boster Scientific Support

Answered: 2015-05-20

Question

Is a blocking peptide available for product anti-Biglycan Monoclonal antibody (M02090)?

P. Singh

Verified customer

Asked: 2015-01-21

Answer

We do provide the blocking peptide for product anti-Biglycan Monoclonal antibody (M02090). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-01-21

Order DetailsPrice
M02090

100μL

$370
M02090-30ul

30μL sample

$99
M02090-carrier-free

Carrier Free

$370

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M02090
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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