Product Info Summary
SKU: | A02459 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Bit1 PTRH2 Antibody
SKU/Catalog Number
A02459
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bit1 PTRH2 Antibody (Catalog # A02459). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Bit1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bit1 PTRH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02459)
Host
Rabbit
Contents
Bit1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bit1 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Bit1. The immunogen is located within amino acids 20 - 70 of Bit1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
Reactive Species
A02459 is reactive to PTRH2 in Human, Mouse, Rat
Applications
A02459 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
19.194kDa
Background of PTRH2
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1
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Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 μg/mL.
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Immunofluorescence of Bit1 in 3T3 cells with Bit1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PTRH2 (Source: Uniprot.org, NCBI)
Gene Name
PTRH2
Full Name
Peptidyl-tRNA hydrolase 2, mitochondrial
Weight
19.194kDa
Superfamily
PTH2 family
Alternative Names
Bcl-2 inhibitor of transcription 1; BIT1CGI-147; EC 3.1.1.29; mitochondrial; peptidyl-tRNA hydrolase 2; PTH2FLJ32471 PTRH2 BIT1, CFAP37, CGI-147, IMNEPD, PTH, PTH 2, PTH2 peptidyl-tRNA hydrolase 2 peptidyl-tRNA hydrolase 2, mitochondrial|bcl-2 inhibitor of transcription 1|cilia and flagella associated protein 37
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTRH2, check out the PTRH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTRH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bit1 PTRH2 Antibody (A02459)
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No publications found for A02459
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