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Product Info Summary
| SKU: | M00313-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-BMI1 Antibody (C-term)
SKU/Catalog Number
M00313-1
Size
50 µl
Description
Boster Bio Anti-BMI1 Antibody (C-term) (Catalog # M00313-1). Tested in WB, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BMI1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00313-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This BMI1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-314 amino acids from the C-terminal region of human BMI1.
Reactive Species
M00313-1 is reactive to BMI1 in Human, Mouse
Calculated molecular weight
36.9 kDa
Background of BMI1
Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00313-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000-1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 with M00313-1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).
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All lanes : Anti-BMI1 Antibody (C-term) at 1:1000-1:2000 dilution
Lane 1: A549 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: mouse thymus lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 37 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-BMI1 Antibody (C-term) (M00313-1)
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