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Product Info Summary
| SKU: | PB9690 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-BMP6 Antibody Picoband®
SKU/Catalog Number
PB9690
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BMP6 Antibody Picoband® catalog # PB9690. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMP6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9690)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human BMP6, different from the related mouse sequence by six amino acids, and from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9690 is reactive to BMP6 in Human
Observed Molecular Weight
43 kDa
Calculated molecular weight
57.2 kDa
Background of BMP6
Bone morphogenetic protein 6 is a protein that in humans is encoded by the BMP6 gene. It is mapped to 6p24-p23. The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9690 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: HELA Whole Cell
FCM: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of BMP6 using anti-BMP6 antibody (PB9690).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP6 antigen affinity purified polyclonal antibody (Catalog # PB9690) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP6 at approximately 43 kDa. The expected band size for BMP6 is at 57 kDa.
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Flow Cytometry analysis of Hela cells using anti-BMP6 antibody (PB9690).
Overlay histogram showing Hela cells stained with PB9690 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BMP6 Antibody (PB9690,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-BMP6 Antibody Picoband® (PB9690)
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Customer Q&As
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4 Customer Q&As for Anti-BMP6 Antibody Picoband®
Question
What are the HELA cells permeabilizated with to produce the flow picture on the PB9690 antibody page?
Verified customer
Asked: 2019-08-21
Answer
The HELA cells used in the Anti-BMP6 Antibody Picoband PB9690 were permeabilizated with saponin.
Boster Scientific Support
Answered: 2019-08-22
Question
I have a quick question about the cells which were used to produced the Flow picture on the product page. Are the HELA cells permeabilisated with MeOH or saponin? Did you used something like that for the cells?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The HELA cells were permeabilisated with saponin.
Boster Scientific Support
Answered: 2019-06-20
Question
My name is Jacqueline and I have a quick question about the cells which were used to produced the Flow picture on the product page. Are the HELA cells permeabilisated with MeOH or saponin? Did you used something like that for the cells?
Verified Customer
Verified customer
Asked: 2015-02-06
Answer
The HELA cells were permeabilisated with saponin.
Boster Scientific Support
Answered: 2015-02-06
Question
We are currently using anti-BMP6 antibody PB9690 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
B. Parker
Verified customer
Asked: 2013-06-17
Answer
The anti-BMP6 antibody (PB9690) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-17
