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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Boster Bio Anti-BNIP3 Antibody catalog # PA1057. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BNIP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1057)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human BNIP3 (38-53aa IYNGDMEKILLDAQHE), identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1057 is reactive to BNIP3 in Human, Mouse, Rat
PA1057 is guaranteed for WB Boster Guarantee
Background of BNIP3
The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3, expressed in skeletal muscle and in the brain at low levels, is primarily localized to the nucleus of glial cells of the normal human brain, as well as in the malignant glioma cell line U251. BNIP3 expression in the cytoplasm increases and localizes with the mitochondria, contributing to induction of cell death. Cellular protein BNIP3 interacts with E1B-19K, BCL-2, BCL-xL, and EBV-BHRF1. BNIP3 contains Bcl-2 homology 3 (BH3) domain and COOH-terminal transmembrane (TM) domain. The BH3 domain of BNIP3 mediates Bcl-2/Bcl-X (L) heterodimerization and confers pro-apoptotic activity; whereas the TM domain is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Anti-BNIP3 antibody, PA1057, Western blotting
All lanes: Anti BNIP3 (PA1057) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MM231Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 30KD
Gene/Protein Information For BNIP3 (Source: Uniprot.Org, NCBI)
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kD-interacting protein 3; BNIP3; Nip3 BNIP3 NIP3 BCL2 interacting protein 3 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3|BCL2/adenovirus E1B 19kDa interacting protein 3|BCL2/adenovirus E1B interacting protein 3|nineteen kD interacting protein-3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on BNIP3, check out the BNIP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BNIP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1057 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao YC,Guo W,Gao BH. Hypoxic training upregulates mitochondrial turnover and angiogenesis of skeletal muscle in mice. Life Sci. 2021 Mar 11:119340.doi:10.1016/j.lfs.2021.119340.Epub ahead of print.PMID:33716067.
PA1057 usage in article: APP:WB, SAMPLE:GASTROCNEMIUS TISSUE, DILUTION:1:1000
Du Y, Li J, Xu T, Zhou DD, Zhang L, Wang X. Oncotarget. 2017 Jun 22;8(37):61510-61527. doi: 10.18632/oncotarget.18604. eCollection 2017 Sep 22. MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling
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