Product Info Summary
| SKU: | A31984 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Brachyury TBXT Antibody
SKU/Catalog Number
A31984
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Brachyury TBXT Antibody catalog # A31984. Tested in WB,ICC/IF applications. This antibody reacts with Human,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Brachyury TBXT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31984)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury(NP_003172.1).
Reactive Species
A31984 is reactive to TBXT in Human, Rat
Calculated molecular weight
47.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31984 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
WesBrachyuryern bloBrachyury analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb aBrachyury 1:1000 diluBrachyuryion.
Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG aBrachyury 1:10000 diluBrachyuryion.
LysaBrachyuryes/proBrachyuryeins: 25ug per lane.
Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.
DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury .
Exposure Brachyuryime: 30s.
Click image to see more details
Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb aBrachyury diluBrachyuryion of 1:200 . Blue: DAPI for nuclear sBrachyuryaining.
Specific Publications For Anti-Brachyury TBXT Antibody (A31984)
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