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Product Info Summary
| SKU: | M00075-4 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-BRAF V600E Antibody(C-Term)
SKU/Catalog Number
M00075-4
Size
50 µl
Description
Boster Bio Anti-BRAF V600E Antibody(C-Term) (Catalog # M00075-4). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BRAF V600E Antibody(C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00075-4)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from human.
Reactive Species
M00075-4 is reactive to BRAF in Human
Calculated molecular weight
84.4 kDa
Background of BRAF
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00075-4 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-BRAF Antibody (C-term) at 1:2000 dilution
Lane 1: Braf-600V -wild type
Lane 2: Braf-600E -mutation
Lane 3: Raji whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: T47D whole cell lysate
Lane 7: MDA-MB-453 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 84 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M00075-4 staining BRAF in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-BRAF V600E Antibody(C-Term) (M00075-4)
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