Anti-Brm SMARCA2 Antibody

BRM antibody

Boster Bio Anti-Brm SMARCA2 Antibody catalog # A01888-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01888-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Brm SMARCA2 Antibody

View all BRM Antibodies

SKU/Catalog Number

A01888-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Brm SMARCA2 Antibody catalog # A01888-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Brm SMARCA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01888-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Brm.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01888-1 is reactive to SMARCA2 in Human, Mouse, Rat

Applications

A01888-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

181.279kDa

Background of BRM

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Babb TL et al.Epilepsy Res. 2005 Mar-Apr;64(1-2):23-30
Chazot PL. Curr Med Chem. 2004 Feb;11(3):389-96
Kakegawa W, et al.Eur J Neurosci. 2003 Feb;17(4):887-91

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
ELISA 1:20000

Validation Images & Assay Conditions

Gene/Protein Information For SMARCA2 (Source: Uniprot.org, NCBI)

Gene Name

SMARCA2

Full Name

Probable global transcription activator SNF2L2

Weight

181.279kDa

Superfamily

SNF2/RAD54 helicase family

Alternative Names

ATP-dependent helicase SMARCA2; BAF190B; BRG1-associated factor 190B; BRMFLJ36757; EC 3.6.1; EC 3.6.4.-; global transcription activator homologous sequence; hBRMMGC74511; hSNF2a; probable global transcription activator SNF2L2; Protein brahma homolog; SNF2; SNF2/SWI2-like protein 2; SNF2A; SNF2-alpha; SNF2L2BAF190; SNF2LA; SNF2-like 2; Sth1p; subfamily a, member 2; sucrose nonfermenting 2-like protein 2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; SWI/SNF-related matrix-associated actin- SMARCA2 BAF190, BRM, NCBRS, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 probable global transcription activator SNF2L2|ATP-dependent helicase SMARCA2|BAF190B|BRG1-associated factor 190B|SNF2-alpha|SNF2/SWI2-like protein 2|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2|brahma homolog|global transcription activator homologous sequence|protein brahma homolog|sucrose nonfermenting 2-like protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SMARCA2, check out the SMARCA2 Infographic

SMARCA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMARCA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Brm SMARCA2 Antibody

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-Brm antibody A01888-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-13

Question

Does anti-Brm antibody A01888-1 work for WB with embryonic kidney?

G. Walker

Verified customer

Asked: 2020-02-13

Answer

According to the expression profile of embryonic kidney, SMARCA2 is highly expressed in embryonic kidney. So, it is likely that anti-Brm antibody A01888-1 will work for WB with embryonic kidney.

Boster Scientific Support

Answered: 2020-02-13

Question

I have attached the WB image, lot number and protocol we used for embryonic kidney using anti-Brm antibody A01888-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-03

Question

We need to test anti-Brm antibody A01888-1 on rat embryonic kidney for research purposes, then I may be interested in using anti-Brm antibody A01888-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

The products we sell, including anti-Brm antibody A01888-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-12

Question

Will anti-Brm antibody A01888-1 work on monkey WB with liver?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

Our lab technicians have not tested anti-Brm antibody A01888-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Brm antibody A01888-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-17

Question

I was wanting to use your anti-Brm antibody for WB for rat embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat embryonic kidney identification?

K. Parker

Verified customer

Asked: 2015-01-02

Answer

You can see on the product datasheet, A01888-1 anti-Brm antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryonic kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-01-02

Question

Will A01888-1 anti-Brm antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Carter

Verified customer

Asked: 2014-02-05

Answer

It shows on the product datasheet, A01888-1 anti-Brm antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-02-05

Order DetailsPrice
A01888-1

100uL

$399

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