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Product Info Summary
| SKU: | A30577 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-BRP44 MPC2 Antibody
SKU/Catalog Number
A30577
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BRP44 MPC2 Antibody (Catalog# A30577). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRP44 MPC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30577)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BRP44. Immunogen sequence location: 31-80
Reactive Species
A30577 is reactive to MPC2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
14.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30577 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using BRP44 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BRP44 MPC2 Antibody (A30577)
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4 Customer Q&As for Anti-BRP44 MPC2 Antibody
Question
We are currently using anti-BRP44 antibody A30577 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The anti-BRP44 antibody (A30577) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-28
Question
Our lab want to know about to test anti-BRP44 antibody A30577 on mouse thalamus for research purposes, then I may be interested in using anti-BRP44 antibody A30577 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The products we sell, including anti-BRP44 antibody A30577, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-11
Question
Would A30577 anti-BRP44 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
As indicated on the product datasheet, A30577 anti-BRP44 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-03
Question
Does anti-BRP44 antibody A30577 work for IHC with thalamus?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
According to the expression profile of thalamus, MPC2 is highly expressed in thalamus. So, it is likely that anti-BRP44 antibody A30577 will work for IHC with thalamus.
Boster Scientific Support
Answered: 2018-02-05
