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Product Info Summary
| SKU: | M00934 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-BST2 Monoclonal Antibody
SKU/Catalog Number
M00934
BM5239 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-BST2 Monoclonal Antibody catalog # M00934. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BST2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00934)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACCD-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BST2 May be involved in the sorting of secreted proteins (By similarity) . May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface.
Reactive Species
M00934 is reactive to BST2 in Human
Observed Molecular Weight
28-40 kDa
Calculated molecular weight
19.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00934 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:20
Positive Control
WB: HeLa cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of BST2 expression in HeLa cell lysate.
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IHC analysis of BST2 using anti-BST2 antibody (M00934).
BST2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BST2 Antibody (M00934) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-BST2 Monoclonal Antibody (M00934)
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Customer Q&As
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6 Customer Q&As for Anti-BST2 Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-BST2 Monoclonal antibody M00934. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
Does anti-BST2 Monoclonal antibody M00934 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
According to the expression profile of liver, BST2 is highly expressed in liver. So, it is likely that anti-BST2 Monoclonal antibody M00934 will work for WB with liver.
Boster Scientific Support
Answered: 2020-04-09
Question
Is this M00934 anti-BST2 Monoclonal antibody reactive to the isotypes of BST2?
H. Carter
Verified customer
Asked: 2020-01-22
Answer
The immunogen of M00934 anti-BST2 Monoclonal antibody is A synthesized peptide derived from human BST2 May be involved in the sorting of secreted proteins (By similarity) . May be involved in pre-B-cell growth. antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-22
Question
Is a blocking peptide available for product anti-BST2 Monoclonal antibody (M00934)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We do provide the blocking peptide for product anti-BST2 Monoclonal antibody (M00934). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-BST2 Monoclonal antibody M00934 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The anti-BST2 Monoclonal antibody (M00934) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-01
Question
See below the WB image, lot number and protocol we used for liver using anti-BST2 Monoclonal antibody M00934. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-10
