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Product Info Summary
| SKU: | A00056 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-C-C motif chemokine 2 CCL2 Antibody
SKU/Catalog Number
A00056
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-C-C motif chemokine 2 CCL2 Antibody (Catalog # A00056). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CCL2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-C-C motif chemokine 2 CCL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00056)
Host
Rabbit
Contents
CCL2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal CCL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human CCL2. The immunogen is located within the last 50 amino acids of CCL2.
Reactive Species
A00056 is reactive to CCL2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
11.0 kDa
Background of CCL2
CCL2, also known as monocyte chemotactic protein 1 (MCP1), belongs to the intercrine beta (chemokine CC) family. It is produced by a variety of cell types and is a potent chemoattractant for monocytes, memory T lymphocytes, and natural killer (NK) cells. It is upregulated during infection and inflammation. CCL2 is a potent basophil activator but does not affect eosinophils, whereas the related protein MCP2 stimulates both eosinophils and basophils. MCP3 has been shown to have the broadest range of influence. CCL2 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00056 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CCL2 antibody can be used for detection of CCL2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 μg/mL.
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Immunohistochemistry of CCL2 in human spleen tissue with CCL2 antibody at 5 μg/ml.
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Immunofluorescence of CCL2 in human spleen tissue with CCL2 antibody at 20 μg/ml.
Specific Publications For Anti-C-C motif chemokine 2 CCL2 Antibody (A00056)
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