Anti-C5/C5a Antibody

Product Name

Anti-C5/C5a Antibody

View all C5 Antibodies

SKU/Catalog Number

PA2308

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-C5/C5a Antibody catalog # PA2308. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C5/C5a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2308)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a, different from the related mouse sequence by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2308 is reactive to C5 in Mouse, Rat

Applications

PA2308 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

115 kDa

Calculated molecular weight

188.305kDa

Background of C5

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

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Anti-C5/C5a antibody, PA2308, IHC(P)
IHC(P): Mouse Spleen Tissue

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Anti-C5/C5a antibody, PA2308, IHC(P)
IHC(P): Rat Liver Tissue

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Anti-C5/C5a antibody, PA2308, IHC(P)
IHC(P): Rat Spleen Tissue

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Anti-C5/C5a antibody, PA2308, All Western blotting
All lanes: Anti-C5a(PA2308) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Predicted bind size: 115KD
Observed bind size: 115KD

Gene/Protein Information For C5 (Source: Uniprot.org, NCBI)

Alternative Names

C5; Complement Component C5/C5a C5 C5Da, C5b, CPAMD4, ECLZB, C5 complement C5 complement C5|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4|C5a anaphylatoxin|anaphylatoxin C5a analog|complement component 5|prepro-C5

For more info on C5, check out the C5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for C5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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