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Product Info Summary
| SKU: | M01010 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01010
BM4064 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody catalog # M01010. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01010)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BAO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human C9
Reactive Species
M01010 is reactive to C9 in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
63.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01010 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: Human placenta tissue
IHC: human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of C9 using anti-C9 antibody (M01010).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9 antigen affinity purified monoclonal antibody (Catalog # M01010) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C9 at approximately 70 kDa. The expected band size for C9 is at 63,70 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using C9 Antibody.
Specific Publications For Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody (M01010)
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1 Customer Q&As for Anti-C9/Complement Component C9 Rabbit Monoclonal Antibody
Question
Is there any difference between M01010 and M01010-1 except for the host and the size?
Verified customer
Asked: 2019-06-13
Answer
The main differences between M01010 and M01010-1 are the hosts and size. Rabbit antibodies usually have higher immunogenicity against human protein targets in comparison with mice and rat antibodies. Rabbit antibodies are also usually more specific and sensitive than mouse antibodies, being ideal for applications like IHC, IF staining and flow cytometry.
Boster Scientific Support
Answered: 2019-06-13
