Click image to see more details
Product Info Summary
| SKU: | A01523-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband®
SKU/Catalog Number
A01523-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband® catalog # A01523-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01523-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CA IV recombinant protein (Position: A19-S284). Human CA IV shares 55.5% and 58.9% amino acid (aa) sequence identity with mouse and rat CA IV, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01523-1 is reactive to CA4 in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
35.0 kDa
Background of CA4
Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01523-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CA IV using anti-CA IV antibody (A01523-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA IV antigen affinity purified polyclonal antibody (Catalog # A01523-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA IV at approximately 35KD. The expected band size for CA IV is at 35KD.
Specific Publications For Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband® (A01523-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband®
Question
Is there a BSA free version of anti-Carbonic Anhydrase IV/CA4 antibody A01523-1 available?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Carbonic Anhydrase IV/CA4 antibody A01523-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-25
Question
We are currently using anti-Carbonic Anhydrase IV/CA4 antibody A01523-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-Carbonic Anhydrase IV/CA4 antibody (A01523-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
Question
Please see the WB image, lot number and protocol we used for lung using anti-Carbonic Anhydrase IV/CA4 antibody A01523-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
