Product Info Summary
SKU: | A30588 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-CA12/Ca Xii Antibody
View all Carbonic Anhydrase XII/CA12 Antibodies
SKU/Catalog Number
A30588
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CA12/Ca Xii Antibody (Catalog# A30588). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CA12/Ca Xii Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30588)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CA12. Immunogen sequence location: 231-280
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30588 is reactive to CA12 in Human, Mouse, Rat
Applications
A30588 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
39.451kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 100-300
Western Blot, 1:500 - 1:2000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from MCF-7 cells, using CA12 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CA12 (Source: Uniprot.org, NCBI)
Gene Name
CA12
Full Name
Carbonic anhydrase 12
Weight
39.451kDa
Superfamily
alpha-carbonic anhydrase family
Alternative Names
CA12; Carbonate dehydratase XII; carbonic anhydrase 12; Carbonic Anhydrase XII; carbonic anhydrase XIICAXII; carbonic dehydratase; CA-XII; EC 4.2.1.1; FLJ20151; HsT18816; Tumor antigen HOM-RCC-3.1.3 CA12 CA-XII, CAXII, HsT18816, T18816 carbonic anhydrase 12 carbonic anhydrase 12|carbonate dehydratase XII|carbonic anhydrase XII|carbonic dehydratase|tumor antigen HOM-RCC-3.1.3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CA12, check out the CA12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CA12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CA12/Ca Xii Antibody (A30588)
Hello CJ!
No publications found for A30588
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Anti-CA12/Ca Xii Antibody
Question
Does anti-CA12/Ca Xii antibody A30588 work for WB with lung?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
According to the expression profile of lung, CA12 is highly expressed in lung. So, it is likely that anti-CA12/Ca Xii antibody A30588 will work for WB with lung.
Boster Scientific Support
Answered: 2020-04-07
Question
I have attached the WB image, lot number and protocol we used for lung using anti-CA12/Ca Xii antibody A30588. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-CA12/Ca Xii antibody A30588. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-11
Question
Will anti-CA12/Ca Xii antibody A30588 work on primate WB with eye kidney?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
Our lab technicians have not validated anti-CA12/Ca Xii antibody A30588 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CA12/Ca Xii antibody A30588 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate eye kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-22
Question
Is this A30588 anti-CA12/Ca Xii antibody reactive to the isotypes of CA12?
J. Edwards
Verified customer
Asked: 2013-06-12
Answer
The immunogen of A30588 anti-CA12/Ca Xii antibody is A peptide derived from human CA12. Immunogen sequence location: 231-280. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-06-12