Click image to see more details
Product Info Summary
| SKU: | A30588 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CA12/Ca Xii Antibody
SKU/Catalog Number
A30588
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CA12/Ca Xii Antibody (Catalog# A30588). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CA12/Ca Xii Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30588)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CA12. Immunogen sequence location: 231-280
Reactive Species
A30588 is reactive to CA12 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
39.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30588 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 100-300.
WB 1:500 - 1:2000.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from MCF-7 cells, using CA12 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using CA12 antibody.
Specific Publications For Anti-CA12/Ca Xii Antibody (A30588)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CA12/Ca Xii Antibody
Question
Does anti-CA12/Ca Xii antibody A30588 work for WB with lung?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
According to the expression profile of lung, CA12 is highly expressed in lung. So, it is likely that anti-CA12/Ca Xii antibody A30588 will work for WB with lung.
Boster Scientific Support
Answered: 2020-04-07
Question
I have attached the WB image, lot number and protocol we used for lung using anti-CA12/Ca Xii antibody A30588. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-CA12/Ca Xii antibody A30588. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-11
Question
Will anti-CA12/Ca Xii antibody A30588 work on primate WB with eye kidney?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
Our lab technicians have not validated anti-CA12/Ca Xii antibody A30588 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CA12/Ca Xii antibody A30588 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate eye kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-22
Question
Is this A30588 anti-CA12/Ca Xii antibody reactive to the isotypes of CA12?
J. Edwards
Verified customer
Asked: 2013-06-12
Answer
The immunogen of A30588 anti-CA12/Ca Xii antibody is A peptide derived from human CA12. Immunogen sequence location: 231-280. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-06-12
