CA3 antibody (PB10049) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 1 Publication
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Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®

CA3 antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitIHC, WB

SKU: PB10049

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

2 in stock. Ships Next Business Day.

Quantity: 1

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Tested Applications

CA3 antibody was positively detected by WB in:

rat liver tissue, rat fat tissue, mouse liver tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CA3 antibody was positively detected by IHC in:

mouse skeletal muscle tissue, rat skeletal muscle tissue, human osteosarcoma tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Recommend Dilution

Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Mouse, Rat, Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
IHC
IF
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Human
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-Carbonic Anhydrase III/CA3 Antibody Picoband®
View all CA3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CA3 recombinant protein (Position: D14-E235). Human CA3 shares 92.3% and 91.4% amino acid (aa) sequence identity with mouse and rat CA3, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight

29.6 kDa

Background of CA3
Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.
Cite This Product
Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10049)

Protocols

Western Blot protocol for CA3 antibody
Immunohistochemistry (IHC) protocol for CA3 antibody

Publications

Application:
Lan F, Yue X, Han L, Shi Z, Yang Y, Pu P, Yao Z, Kang C. Int J Oncol. 2012 Feb;40(2):519-26. Doi: 10.3892/Ijo.2011.1215. Epub 2011 Sep 28. Genome-Wide Identification Of Tcf7L2/Tcf4 Target Mirnas Reveals A Role For Mir-21 In Wnt-Driven Epithelial C...
PMID: 21956205

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Product Promise

We promise Anti-Carbonic Anhydrase III/CA3 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PB10049
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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Guaranteed product quality

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Sep 22, 2026