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Product Info Summary
| SKU: | A00463-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CAD/CAD Antibody Picoband®
SKU/Catalog Number
A00463-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CAD/CAD Antibody Picoband® catalog # A00463-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAD/CAD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00463-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CAD recombinant protein (Position: L1555-K1780).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00463-1 is reactive to CAD in Human, Mouse
Observed Molecular Weight
270 kDa
Calculated molecular weight
243.0 kDa
Background of CAD
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This CAD gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00463-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse pancreas tissue
Validation Images & Assay Conditions
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Western blot analysis of CAD using anti-CAD antibody (A00463-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse pancreas tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAD antigen affinity purified polyclonal antibody (Catalog # A00463-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAD at approximately 270KD. The expected band size for CAD is at 243KD.
Specific Publications For Anti-CAD/CAD Antibody Picoband® (A00463-1)
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4 Customer Q&As for Anti-CAD/CAD Antibody Picoband®
Question
I was wanting to use to test anti-CAD/CAD antibody A00463-1 on mouse colon adenocarcinoma hepatoma for research purposes, then I may be interested in using anti-CAD/CAD antibody A00463-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The products we sell, including anti-CAD/CAD antibody A00463-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-31
Question
I see that the anti-CAD/CAD antibody A00463-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-06
Question
Will anti-CAD/CAD antibody A00463-1 work for WB with colon adenocarcinoma hepatoma?
O. Rodriguez
Verified customer
Asked: 2016-07-08
Answer
According to the expression profile of colon adenocarcinoma hepatoma, CAD is highly expressed in colon adenocarcinoma hepatoma. So, it is likely that anti-CAD/CAD antibody A00463-1 will work for WB with colon adenocarcinoma hepatoma.
Boster Scientific Support
Answered: 2016-07-08
Question
We are currently using anti-CAD/CAD antibody A00463-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
M. Williams
Verified customer
Asked: 2015-04-06
Answer
The anti-CAD/CAD antibody (A00463-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-06
