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Product Info Summary
| SKU: | A05374 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CAD Dffb Antibody
SKU/Catalog Number
A05374
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CAD Dffb Antibody (Catalog# A05374). Tested in IHC, ICC, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAD Dffb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05374)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CAD. Immunogen sequence location: 289 - 338
Reactive Species
A05374 is reactive to Dffb in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
39.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05374 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-200
WB 1:500-2000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night
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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night
Specific Publications For Anti-CAD Dffb Antibody (A05374)
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Customer Q&As
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4 Customer Q&As for Anti-CAD Dffb Antibody
Question
We have observed staining in human cerebellar hemisphere. What should we do? Is anti-CAD antibody supposed to stain cerebellar hemisphere positively?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
From what I have seen in literature cerebellar hemisphere does express DFFB. From what I have seen in Uniprot.org, DFFB is expressed in cerebellar hemisphere, pancreas, fetal brain, lung, among other tissues. Regarding which tissues have DFFB expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Pancreas, Pubmed ID: 9560346
Boster Scientific Support
Answered: 2020-04-30
Question
We are currently using anti-CAD antibody A05374 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The anti-CAD antibody (A05374) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
Question
We were content with the WB result of your anti-CAD antibody. However we have observed positive staining in pancreas cytoplasm. nucleus. using this antibody. Is that expected? Could you tell me where is DFFB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
From literature, pancreas does express DFFB. Generally DFFB expresses in cytoplasm. nucleus. Regarding which tissues have DFFB expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Pancreas, Pubmed ID: 9560346
Boster Scientific Support
Answered: 2019-05-21
Question
Our lab want to know about using your anti-CAD antibody for protein homooligomerization studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.
K. Lewis
Verified customer
Asked: 2018-12-05
Answer
I appreciate your inquiry. This A05374 anti-CAD antibody is tested on jurkat cells. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-05
