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Product Info Summary
| SKU: | A10388 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Cadherin-7 CDH7 Antibody
SKU/Catalog Number
A10388
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cadherin-7 CDH7 Antibody catalog # A10388. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cadherin-7 CDH7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10388)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CDH7. AA range:651-700
Reactive Species
A10388 is reactive to CDH7 in Human, Mouse, Rat
Calculated molecular weight
87.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10388 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Cadherin-7 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from A549 cells, using CDH7 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Cadherin-7 CDH7 Antibody (A10388)
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Customer Q&As
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5 Customer Q&As for Anti-Cadherin-7 CDH7 Antibody
Question
We are currently using anti-Cadherin-7 antibody A10388 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Cadherin-7 antibody (A10388) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this A10388 anti-Cadherin-7 antibody reactive to the isotypes of CDH7?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The immunogen of A10388 anti-Cadherin-7 antibody is Synthesized peptide derived from the C-terminal region of human Cadherin-7. at AA rangle: 620-700. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-07
Question
Does anti-Cadherin-7 antibody A10388 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
According to the expression profile of kidney, CDH7 is highly expressed in kidney. So, it is likely that anti-Cadherin-7 antibody A10388 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-08-08
Question
My question regarding product A10388, anti-Cadherin-7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10388 anti-Cadherin-7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-17
Question
Would anti-Cadherin-7 antibody A10388 work on goat WB with kidney?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
Our lab technicians have not tested anti-Cadherin-7 antibody A10388 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Cadherin-7 antibody A10388 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-25
