SKU M03047-2
Size 100ul
Reactivity Bovine, Human, Mouse, Rat
Clonality Polyclonal
Host Chicken
Applications IF, IHC, WB

Overview

Product Name Anti-Calbindin Antibody
SKU/Catalog Number M03047-2
Storage & Handling Store at 4℃ for short term and -20℃ for long term. Avoid repeated freeze / thaw cycles.
Size 100ul
Description Anti-Calbindin Antibody tested in WB, IF, ICC, IHC, reactive to Human, cow, rat, mouse
Cite This Product Anti-Calbindin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047-2)
Host Chicken
Contents/Buffer Supplied as an aliquot of IgY preparation plus 5mM NaN3
Immunogen Full-length recombinant human protein
Reactivity Bovine, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB: 1:1,000-1:5,000. IF/ICC or IHC: 1:1,000-1:5,000.

Images And Assay Conditions

Western blot analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, M03047-2, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. The M03047-2 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.

Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to calbindin, M03047-2, dilution 1:2,000, in green, and costained with rabbit pAb to MeCP2, dilution 1:5,000, in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45?M, and free-floating sections were stained with above antibodies. Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05937
Gene Name CALB1
Protein Name Calbindin
Alternative Names Calbindin;Calbindin D28;D-28K;Vitamin D-dependent calcium-binding protein, avian-type;CALB1;CAB27;
Molecular Weight 30025 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Research Areas Bovine, Human, Mouse, Rat

*You can search these to find other products in these research areas.

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Anti-Calbindin Antibody tested in WB, IF, ICC, IHC, reactive to Human, cow, rat, mouse
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In stock
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M03047-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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