Anti-Calbindin Monoclonal Antibody

Boster Bio Anti-Calbindin Monoclonal Antibody catalog # M03047-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Product Info Summary

SKU: M03047-1
Size: 100ul
Reactive Species: Bovine, Human, Mouse, Pig, Rat
Host: Mouse
Application: IF, IHC, WB

Product Name

Anti-Calbindin Monoclonal Antibody

See all Calbindin D-28K products

SKU/Catalog Number

M03047-1

Size

100ul

Description

Boster Bio Anti-Calbindin Monoclonal Antibody catalog # M03047-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calbindin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047-1)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

Clonality

Monoclonal

Immunogen

Full-length recombinant human calbindin protein expressed in and purified from E.Coli.

Reactive Species

M03047-1 is reactive to CALB1 in Bovine, Human, Mouse, Pig, Rat

Applications

M03047-1 is guaranteed for IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:5,000. ICC/IF or IHC: 1:1,000.

Validation Images & Assay Conditions

Gene/Protein Information For CALB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05937

Gene ID

793

Gene Name

CALB1

Full Name

Calbindin

Weight

30.025kDa

Superfamily

calbindin family

Alternative Names

CAB27; CALB; calbindin 1, (28kD); calbindin 1, 28kDa; Calbindin D28; calbindin; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type CALB1 CALB, D-28K calbindin 1 calbindin|RTVL-H protein|calbindin 1, (28kD)|calbindin 1, 28kDa|calbindin 27|calbindin D28|vitamin D-dependent calcium-binding protein, avian-type

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CALB1, check out the CALB1 Infographic

CALB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CALB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M03047-1

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15 Customer Q&As for Anti-Calbindin Monoclonal Antibody

Question

Is this M03047-1 anti-Calbindin Monoclonal antibody reactive to the isotypes of CALB1?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

The immunogen of M03047-1 anti-Calbindin Monoclonal antibody is Full-length recombinant human calbindin protein expressed in and purified from E.Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-26

Question

We have observed staining in rat caudate nucleus. Are there any suggestions? Is anti-Calbindin Monoclonal antibody supposed to stain caudate nucleus positively?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

From literature caudate nucleus does express CALB1. From Uniprot.org, CALB1 is expressed in caudate nucleus, brain, amygdala, cerebellum substantia nigra, kidney lung, among other tissues. Regarding which tissues have CALB1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cerebellum, and Substantia nigra, Pubmed ID: 14702039
Brain, Pubmed ID: 2565876, 3691519
Kidney, and Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-30

Question

See below the WB image, lot number and protocol we used for amygdala using anti-Calbindin Monoclonal antibody M03047-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-17

Question

Can you help my question with product M03047-1, anti-Calbindin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03047-1 anti-Calbindin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-31

Question

We want to test anti-Calbindin Monoclonal antibody M03047-1 on human amygdala for research purposes, then I may be interested in using anti-Calbindin Monoclonal antibody M03047-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

The products we sell, including anti-Calbindin Monoclonal antibody M03047-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-28

Question

Our lab used your anti-Calbindin Monoclonal antibody for WB on cerebellum substantia nigra in a previous project. I am using bovine, and We want to use the antibody for IHC next. My lab would like examining cerebellum substantia nigra as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-09-04

Answer

I have checked the website and datasheets of our anti-Calbindin Monoclonal antibody and it appears that M03047-1 has been validated on bovine in both WB and IHC. Thus M03047-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-09-04

Question

I am looking for using your anti-Calbindin Monoclonal antibody for amyloid fiber formation studies. Has this antibody been tested with western blotting on neuronal tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-07-16

Answer

Thanks for your inquiry. This M03047-1 anti-Calbindin Monoclonal antibody is tested on neuronal tissue, purkinje cells, granule cells. It is guaranteed to work for IF, IHC, WB in bovine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-07-16

Question

We are currently using anti-Calbindin Monoclonal antibody M03047-1 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says bovine, human, mouse, pig, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-04

Answer

The anti-Calbindin Monoclonal antibody (M03047-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-Calbindin Monoclonal antibody M03047-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-10-04

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-04

Question

Would M03047-1 anti-Calbindin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

F. Krishna

Verified customer

Asked: 2017-08-11

Answer

It shows on the product datasheet, M03047-1 anti-Calbindin Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-08-11

Question

Would anti-Calbindin Monoclonal antibody M03047-1 work for IHC with amygdala?

Verified Customer

Verified customer

Asked: 2017-06-08

Answer

According to the expression profile of amygdala, CALB1 is highly expressed in amygdala. So, it is likely that anti-Calbindin Monoclonal antibody M03047-1 will work for IHC with amygdala.

Boster Scientific Support

Answered: 2017-06-08

Question

I was wanting to use your anti-Calbindin Monoclonal antibody for IHC for human amygdala on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amygdala identification?

Verified Customer

Verified customer

Asked: 2017-06-05

Answer

You can see on the product datasheet, M03047-1 anti-Calbindin Monoclonal antibody has been validated for IF, IHC, WB on bovine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-05

Question

Is a blocking peptide available for product anti-Calbindin Monoclonal antibody (M03047-1)?

K. Singh

Verified customer

Asked: 2016-10-14

Answer

We do provide the blocking peptide for product anti-Calbindin Monoclonal antibody (M03047-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-10-14

Question

I see that the anti-Calbindin Monoclonal antibody M03047-1 works with IHC, what is the protocol used to produce the result images on the product page?

N. Krishna

Verified customer

Asked: 2013-04-10

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-04-10

Question

Do you have a BSA free version of anti-Calbindin Monoclonal antibody M03047-1 available?

P. Walker

Verified customer

Asked: 2013-03-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Calbindin Monoclonal antibody M03047-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-03-05

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Ships directly to USA and Canada. For other regions, visit our distributors page

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M03047-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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