|Validated Species:||Bovine, Human, Rat|
Data & Images
|Product Name||Anti-Calcineurin Alpha Antibody (Monoclonal, CN-A1)|
|Description||Mouse IgG monoclonal antibody for Calcineurin alpha, protein phosphatase 3, regulatory subunit B, alpha (PPP3R1) detection. Tested with WB, IHC-P in Human;bovine;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Calcineurin Alpha Antibody (Monoclonal, CN-A1) CN-A1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1008)|
|Replacement Item||This antibody may replace the following items: sc-130393|sc-135349|sc-169808|sc-169810|sc-33166|sc-373803|sc-376734|sc-393131|sc-6119 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human, Rat|
|Predicted Species||Bovine, Canine, Chicken, Monkey|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Bovine brain calcineurin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 50% glycerol, 1.2% Sodium acetate, 1% BSA, 0.02% NaN3.|
|Storage||At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Calcineurin subunit B type 1|
|Molecular Weight||19300 MW|
|Protein Function||Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.|
|Tissue Specificity||Isoform 2 is testis specific. .|
|Sequence Similarities||Belongs to the calcineurin regulatory subunit family.|
|Subcellular Localization||Cytoplasm, cytosol . Cell membrane ; Lipid-anchor . Cell membrane, sarcolemma. Translocates from the cytosol to the sarcolemma in a CIB1- dependent manner during cardiomyocytes hypertrophy. .|
|Alternative Names||Calcineurin subunit B type 1;Protein phosphatase 2B regulatory subunit 1;Protein phosphatase 3 regulatory subunit B alpha isoform 1;Ppp3r1;Cna2, Cnb;|
Background for Calcineurin subunit B type 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Calcineurin Alpha Antibody (Monoclonal, CN-A1) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rat, By Heat
Western blot, 0.25-0.5μg/ml, Human, bovine, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.