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- Table of Contents
CNN1 antibody (PA2224) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Anti-Calponin antibody, PA2224, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
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Anti-Calponin/CNN1 Antibody Picoband®
CNN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
CNN1 antibody was positively detected by WB in:
HELA Whole Cell, JURKAT Whole Cell, MCF-7 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CNN1 antibody was positively detected by IHC in:
Human Placenta Tissue, mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Mouse
Tested
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-Calponin/CNN1 Antibody Picoband®
View all CNN1 Antibodies
View all CNN1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Calponin, different from the related mouse and rat sequences by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated Molecular Weight
33.2 kDa
Background of CNN1
Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. This gene is mapped to 19p13.2. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
Cite This Product
Anti-Calponin/CNN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2224)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Dog
93%
High Homology
Mouse
87%
Moderate Homology
Rat
87%
Moderate Homology
Zebrafish
7%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CNN1 antibody
Immunohistochemistry (IHC) protocol for CNN1 antibody
Publications
Application:
Species:
CircLMBR1 inhibits phenotypic transformation of hypoxia-induced pulmonary artery smooth muscle via the splicing factor PUF60
Published: 2024-07-25
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.20
Species: Human,Mouse
PMID: 39059570
Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling
Published: 2018-11-21
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: IF
Impact Factor: 5.50
Species: Rat,Human
PMID: 30463073
Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin‑angiotensin system activity
Published: 2018-09-13
Journal: Experimental and Therapeutic Medicine
Application: IHC,WB
Impact Factor: 1.41
Species: Rat
PMID: 30402152
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Product Promise
We promise Anti-Calponin/CNN1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA2224
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026