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- Table of Contents
CNN1 antibody (M08065-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of Calponin using anti-Calponin antibody (M08065-1).
Calponin was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Calponin Antibody (M08065-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Calponin was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Calponin Antibody (M08065-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Calponin CNN1 Rabbit Monoclonal Antibody
CNN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
CNN1 antibody was positively detected by IHC in:
human appendicitis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Recommend
Tested
Recommend
Recommend
Mouse
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Calponin CNN1 Rabbit Monoclonal Antibody
View all CNN1 Antibodies
View all CNN1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BCC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calponin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
33.2 kDa
Cite This Product
Anti-Calponin CNN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08065-1)
Protocols
Immunohistochemistry (IHC) protocol for CNN1 antibody
Publications
Application:
Species:
FPR2 Agonism Attenuates Restenosis by Mitigating Neointimal Hyperplasia via ELOVL6
Published: 2025-09-06
Journal: FASEB JOURNAL
Application: WB
Impact Factor: 4.20
Species: Mouse
PMID: 40913417
CircLMBR1 inhibits phenotypic transformation of hypoxia-induced pulmonary artery smooth muscle via the splicing factor PUF60
Published: 2024-07-25
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.20
Species: Human,Mouse
PMID: 39059570
Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system
Published: 2020-05-15
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.79
Species: Rat
PMID: 32742325
Review
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Product Promise
We promise Anti-Calponin CNN1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M08065-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026